Methods for measuring hydrophobicity of microorganisms

Mozes, Nava; Rouxhet, Paul

doi:10.1016/0167-7012(87)90058-3

Cited by 213 publications

(113 citation statements)

References 9 publications

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“…This was not confirmed by the MATH data. The lack of agreement between water contact angles and MATH data has also been reported by others (29)(30)(31). Strains with a high ARR value, had a low MATH value whereas strains with a low ARR value had a relatively high MATH value, except for strain O8K(A)28.…”

Section: Cell Biophysicssupporting

confidence: 67%

Physicochemical characterization ofEscherichia coli

et al. 1992

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Eight Escherichia coli strains were characterized by determining their adhesion to xylene, surface free energy, zeta potential, relative surface charge, and their chemical composition. The latter was done by applying X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy OR). No relationship between the adhesion to xylene and the water contact angles of these strains was found. Three strains had significantly lower surface free energies than the other strains. Surface free energies were either obtained from polar and dispersion parts or from Lifshitz-van der Waals and acid/base parts of the surface free energy. A correlation (r = 0.97) between the polar parts and the electron-donor contributions to the acid/base part of the surface free energy was found. The zeta potentials of all strains, measured as a function of pH *Author to whom all correspondence and reprint requests should be addressed. Cell Biophysics 1 7Volume 20, 1992 18Harkes et al.(2-11), were negative. Depending on the zeta potential as a function of pH, three groups were recognized among the strains tested. A relationship (r = 0.84) was found between the acid/base component of the surface free energy and the zeta potential measured at pH = 7.4. There was no correlation between results of XPS and IR studies. Data from the literature of XPS and IR studies of the gram-positive staphylococci and streptococci were compared with data from the gram-negative E. coli used in this study. It appeared that in these three groups of bacteria, the polysaccharide content detected by IR corresponded well with the oxygen-to-carbon ratio detected by XPS.

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Section: Cell Biophysicssupporting

confidence: 67%

Physicochemical characterization ofEscherichia coli

et al. 1992

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“…E -ma il acldre s s : chimbiointlus @fsagx.ac.be (M. PaquoÙ. 0167-'7012/01/$ -see front matter @2001 Elsevier Science B.V. All rights reserved PII: S0 1 67-70 I 2(0 1 ) upon salt addition and latex pafticle agglutination (Dillon et al, 1986;Mozes and Rouxhet, 1987; der Mei et al, 1987) are clearly depending on electrostatic interactions. The same objective holds for adhesion to polystyrene; the surface of polystyrene materials is indeed negatively charged (Dewez et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis

Ahimou

Paquot

Jacques

et al. 2001

Journal of Microbiological Methods

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“…The hydrophobicity of the cells was estimated by measurement of the water contact angle on a lawn of cells (Mozes and Rouxhet, 1987). A lawn of washed cells, about 30 layers thick, was prepared on a ®lter (4.5 cm diameter) with pore diameter 0.45 mm (MF-type, HAWP, Millipore).…”

Section: Contact Angle Measurementmentioning

confidence: 99%

Modification of the aggregation behaviour of the environmental Ralstonia eutropha‐like strain AE815 is reflected by both surface hydrophobicity and amplified fragment length polymorphism (AFLP) patterns

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Huys

et al. 2000

Environmental Microbiology

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SummaryAfter inoculation of the plasmid-free non-aggregative Ralstonia eutropha-like strain AE815 in activated sludge, followed by reisolation on a selective medium, a mutant strain A3 was obtained, which was characterized by an autoaggregative behaviour. Strain A3 had also acquired an IncP1 plasmid, pLME1, co-aggregated with yeast cells when co-cultured, and stained better with Congo red than did the AE815 strain. Contact angle measurements showed that the mutant strain was considerably more hydrophobic than the parent strain AE815, and scanning electron microscopy (SEM) revealed the production of an extracellular substance. A similar hydrophobic mutant (AE176R) could be isolated from the AE815-isogenic R. eutropha-like strain AE176. With the DNA ®ngerprinting technique repetitive extragenic palindromic-polymerase chain reaction (REP-PCR), no differences between these four strains, AE815, A3, AE176 and AE176R, could be revealed. However, using the ampli®ed fragment length polymorphism (AFLP) DNA ®ngerprinting technique with three different primer combinations, small but clear reproducible differences between the banding patterns of the autoaggregative mutants and their non-autoaggregative parent strains were observed for each primer set. These studies demonstrate that,

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Methods for measuring hydrophobicity of microorganisms

Cited by 213 publications

References 9 publications

Physicochemical characterization ofEscherichia coli

Physicochemical characterization ofEscherichia coli

Influence of electrical properties on the evaluation of the surface hydrophobicity of Bacillus subtilis

Modification of the aggregation behaviour of the environmental Ralstonia eutropha‐like strain AE815 is reflected by both surface hydrophobicity and amplified fragment length polymorphism (AFLP) patterns

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