Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria

Penrose, Donna M.; Glick, Bernard R.

doi:10.1034/j.1399-3054.2003.00086.x

Cited by 1,278 publications

(831 citation statements)

References 36 publications

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“…The isolates were further screened for their ACC deaminase activity with and without salt supplementation, by measuring the amount of a-ketobutyrate produced by ACC using 2,4-dinitrophenylhydrazine reagent (Honma and Shimomura 1978;Penrose and Glick 2003). The bacterial cells were grown in a tryptone soya broth up to late log phase and later grown and induced in minimal media (with and without supplemented salt) with additional 3.0 mM ACC as sole nitrogen source.…”

Section: Assessment Of Salt Tolerance Of Isolatesmentioning

confidence: 99%

Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury

Panwar

Tewari

Nayyar

2016

Physiol Mol Biol Plants

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The beneficial microbial-plant interaction plays important role in the soil health, crop growth and productivity. Plant growth promoting rhizobacteria (PGPR) are such beneficial microorganisms, which in association with plant roots not only promote their growth but also help in counteracting the detrimental effects of soil stresses. Salt stress is one such stress, frequently confronted by the plants. The present study aimed at isolation and identification of PGPR inhabiting the mungbean rhizosphere, testing them for salt (NaCl) tolerance and subsequently in salt-supplemented mungbean crop. For this purpose, two salt-tolerant bacterial strains belonging to genus Pantoea and Enterococcus, characterized for their P-solubilization ability, indole acetic acid and siderophore production were selected. These two PGPR were further evaluated for their effect on the salt-stressed mungbean plants, grown at two salt concentrations (5 and 10 dS/m). The plants treated with the combination of PGPR showed better performance in growth (16-37 %) and yield (22-32 %), under salt stress, as compared with control. The increasing salt concentration was found to increase the membrane damage, Na ? concentration in the plants. PGPR treatments effectively reduced the Na ? concentration (17-41 %), membrane damage (1.1-1.5 folds) and enhanced the antioxidants i.e. ascorbic acid (8-26 %) and glutathione (10-30 %) in salt-stressed plants, in comparison to uninoculated stressed plants. Overall, the results indicated that both PGPR were effective as stress mitigators however, in combination they showed relatively better improvement in growth, yield as well as oxidative parameters of the salt-affected plants. These findings about the effects of native salt-tolerant PGPR Pantoea and Enterococcus sp. in mungbean crop are novel.

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Section: Assessment Of Salt Tolerance Of Isolatesmentioning

confidence: 99%

Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury

Panwar

Tewari

Nayyar

2016

Physiol Mol Biol Plants

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“…The ability of the isolates to produce ACC deaminase was also screened on minimal media containing ACC as their sole nitrogen source as described by Penrose and Glick (2003). Optical density (OD) was measured after 48 h at 540 nm by spectrophotometer (Specord 200, Analytic Jena, Germany) and considered as an index for evaluating ACC deaminaseproducing isolates.…”

Section: Isolation Of Endophytic Bacterial Strainsmentioning

confidence: 99%

Bacterial biosynthesis of 1-aminocyclopropane-1-caboxylate (ACC) deaminase, a useful trait to elongation and endophytic colonization of the roots of rice under constant flooded conditions

Etesami

Hosseini

Alikhani

2014

Physiol Mol Biol Plants

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This study was conducted to investigate the role of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in Pseudomonas fluorescens strain REN 1 and its ability to reduce ethylene levels produced during stress, endophytically colonize and promote the elongation of the roots of rice seedlings under gnotobiotic conditions. We isolated 80 bacteria from inside roots of rice plants grown in the farmers' fields in Guilan, Iran. All of the isolates were characterized for plant growth promoting (PGP) traits. In addition, the colonization assay of these isolates on rice seedlings was carried out to screen for competent endophytes. The best bacterial isolate, based on ACC deaminase production, was identified and used for further study. 16S rDNA sequence analysis revealed that the endophyte was closely related to Pseudomonas fluorescens. The results of this study showed ACC deaminase containing P. fluorescens REN1 increased in vitro root elongation and endophytically colonized the root of rice seedlings significantly, as compared to control under constant flooded conditions. The trait of low amount of indole-3-acetic acid (IAA) production (<15 μg mL −1 ) and the high production of ACC deaminase by bacteria may be main factors in colonizing rice seedling roots compared to other PGP traits (siderophore production and phosphate solubilization) in this study. Endophytic IAA and ACC deaminase-producing bacteria may be preferential selections by rice seedlings. Therefore, it may be suggested that the utilization of ACC as a nutrient gives the isolates advantages in more endophytic colonization and increase of root length of rice seedlings.

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“…Growth was determined by measuring the OD at 600 nm after incubation at 30°C for 72 h. The ACCD activity of cell-free extracts was determined by monitoring the amount of a-ketobutyrate generated by the hydrolysis of ACC (Saleh and Glick 2001), by comparing the absorbance (540 nm) of a sample to a standard curve of a-ketobutyrate ranging between 0.1 and 1.0 mmol L -1 according to Penrose and Glick (2003) and Dell'Amico et al (2008). The mixtures containing no cell suspension or no ACC were used as controls.…”

Section: Acc Deaminase Activitymentioning

confidence: 99%

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

Cavalca

Corsini

Bachate

et al. 2013

World J Microbiol Biotechnol

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In the present study, six arsenic-resistant strains previously isolated were tested for their plant growth promoting characteristics and heavy metal resistance, in order to choose one model strain as an inoculum for sunflower plants in pot experiments. The aim was to investigate the effect of arsenic-resistant strain on sunflower growth and on arsenic uptake from arsenic contaminated soil. Based on plant growth promoting characteristics and heavy metal resistance, Alcaligenes sp. strain Dhal-L was chosen as an inoculum. Beside the ability to reduce arsenate to arsenite via an Ars operon, the strain exhibited 1-amino-cyclopropane-1-carboxylic acid deaminase activity and it was also able to produce siderophore and indole acetic acid. Pot experiments were conducted with an agricultural soil contaminated with arsenic (214 mg kg -1 ). A real time PCR method was set up based on the quantification of ACR3(2) type of arsenite efflux pump carried by Alcaligenes sp. strain Dhal-L, in order to monitor presence and colonisation of the strain in the bulk and rhizospheric soil. As a result of strain inoculation, arsenic uptake by plants was increased by 53 %, whereas ACR3(2) gene copy number in rhizospheric soil was 100 times higher in inoculated than in control pots, indicating the colonisation of strain. The results indicated that the presence of arsenate reducing strains in the rhizosphere of sunflower influences arsenic mobilization and promotes arsenic uptake by plant.

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Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria

Cited by 1,278 publications

References 36 publications

Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury

Native halo-tolerant plant growth promoting rhizobacteria Enterococcus and Pantoea sp. improve seed yield of Mungbean (Vigna radiata L.) under soil salinity by reducing sodium uptake and stress injury

Bacterial biosynthesis of 1-aminocyclopropane-1-caboxylate (ACC) deaminase, a useful trait to elongation and endophytic colonization of the roots of rice under constant flooded conditions

Rhizosphere colonization and arsenic translocation in sunflower (Helianthus annuus L.) by arsenate reducing Alcaligenes sp. strain Dhal-L

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