Methods for in vitro modeling of glioma invasion: Choosing tools to meet the need

Dundar, Bilge; Markwell, Steven M.; Sharma, Nikhil; Olson, Cheryl L.; Mukherjee, Subhas; Brat, Daniel J.

doi:10.1002/glia.23813

Cited by 12 publications

(8 citation statements)

References 155 publications

(218 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are many types of hydrogel, such as poly(ɛ-caprolactone)-poly(ethylene glycol) (PCL-PEG), hyaluronic acid, poly(ɛ-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ɛ-caprolactone-co-lactide) (PCLA-PEG-PCLA), and poly(vinyl alcohol) (PVA) [ 15 , 16 , 17 , 18 , 19 , 20 , 24 , 32 , 33 ]. Natural products such as gelatin, triglycerol monostearate, pig diesel, and collagen have been employed as hydrogel scaffolds [ 25 , 27 , 29 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Hydrogel on a Smart Nanomaterial Interface to Carry Therapeutics for Digitalized Glioma Treatment

Zhao

Javed

Tian

et al. 2022

Gels

View full text Add to dashboard Cite

Glioma is considered the primary brain tumor to cause brain illnesses, and it is difficult to treat and shows resistance to various routine therapeutics. The most common treatments to cure glioma are the surgical removal of tumors followed by adjuvant chemotherapy and radiation therapy. The latest biocompatible interfaces have been incorporated into therapeutic modalities such as the targeted delivery of drugs using hydrogels to treat and manage brain glioma. This review illustrates the applications of the multimodal hydrogel as the carrier of therapeutics, gene therapy, therapeutic tactics, and glioma devices. The scientific articles were retrieved from 2019 to 2022 on Google Scholar and the Scopus database and screened to determine whether they were suitable for review. The 20 articles that fit the study are summarized in this review. These studies indicated that the sizes of the hydrogel range from 28 nm to 500 nm. There are 16 out of 20 articles that also explain the post-surgical application of hydrogels, and 13 out of 20 articles are employed in 3D culture and other structural manifestations of hydrogels. The pros of the hydrogel include the quick formulation for a sufficient filling of irregular damage sites, solubilizing hydrophobic drugs, continuously slowing drug release, provision of a 3D cell growth environment, improving efficacy, targetability of soluble biomolecules, increasing patient compliance, and decreased side effects. The cons of the hydrogel include difficult real-time monitoring, genetic manipulations, the cumbersome synchronized release of components, and lack of safety data. The prospects of the hydrogel may include the development of electronic hydrogel sensors that can be used to enhance guidance for the precise targeting patterns using patient-specific pathological idiosyncrasies. This technology has the potential to revolutionize the precision medicine approaches that would aid in the early detection and management of solid brain tumors.

show abstract

Section: Resultsmentioning

confidence: 99%

Hydrogel on a Smart Nanomaterial Interface to Carry Therapeutics for Digitalized Glioma Treatment

Zhao

Javed

Tian

et al. 2022

Gels

View full text Add to dashboard Cite

show abstract

“…Next, we investigated functions for Mlc1 in GSC invasion through three-dimensional ECM [ 23 ]. Spheroids were dissociated and cells were exposed to a serum gradient to induce directional invasion in an ECM-coated transwell system.…”

Section: Resultsmentioning

confidence: 99%

Megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1) promotes glioblastoma cell invasion in the brain microenvironment

Lattier

Chen

et al. 2020

Oncogene

View full text Add to dashboard Cite

Glioblastoma (GBM), or grade IV astrocytoma, is a malignant brain cancer that contains sub-populations of proliferative and invasive cells that coordinately drive tumor growth, progression and recurrence after therapy. Here, we have analyzed functions for megalencephalic leukoencephalopathy with subcortical cysts 1 (Mlc1), an eight-transmembrane protein normally expressed in perivascular brain astrocyte endfeet that is essential for neurovascular development and physiology, in the pathogenesis of GBM. We show that Mlc1 is expressed in human stem-like GBM cells (GSCs) and is linked to the development of primary and recurrent GBM. Genetically inhibiting MLC1 in GSCs using RNAi-mediated gene silencing results in diminished growth and invasion in vitro as well as impaired tumor initiation and progression in vivo. Biochemical assays identify the receptor tyrosine kinase Axl and its intracellular signaling effectors as important for MLC1 control of GSC invasive growth. Collectively, these data reveal key functions for MLC1 in promoting GBM cell growth and invasion, and suggest that targeting the Mlc1 protein or its associated signaling effectors may be a useful therapy for blocking tumor progression in patients with primary or recurrent brain cancer.

show abstract

“…8). Next, we investigated functions for hepaCAM in GSC invasion in vitro (21). Spheroids were dissociated and cells were exposed to a serum gradient to induce directional invasion through an ECM-coated transwell.…”

Section: Hepacam Is Differentially Expressed In Gbm Versus Non-cancer...mentioning

confidence: 99%

HepaCAM Suppresses Glioblastoma Stem Cell Invasion in the Brain

Lattier

Morales

et al. 2022

Preprint

View full text Add to dashboard Cite

Glioblastoma (GBM) is a malignant brain cancer that contains sub-populations of highly invasive tumor cells that drive progression and recurrence after surgery and radiochemotherapy. The exact mechanisms that enable GBM cells to disperse from the main tumor mass and navigate throughout the brain microenvironment remain largely unknown. As a result, there is a lack of effective strategies to block cancer cell invasive growth in primary and recurrent GBM. Here we report that hepatocyte cell adhesion molecule (hepaCAM), which is normally expressed in perivascular astrocytes, plays central roles in controlling the invasive growth features of GBM cells. Genetically targeting HEPACAM induces a transition from GBM cell proliferation/self-renewal to invasion. Increased invasion is due, in part, to an activation of focal adhesion signaling pathways and enhanced GBM cell adhesion to the extracellular matrix (ECM) in the brain microenvironment. Transcriptional profiling of GBM cells reveals various HEPACAM-regulated genes with links to polarity and invasion. Collectively, these data show that hepaCAM balances ECM adhesion and signaling pathways to control cancer cell proliferation versus invasion in the brain parenchyma. Targeting select components of the hepaCAM pathway may be an effective way to block tumor progression and recurrence in patients with GBM.

show abstract

Methods for in vitro modeling of glioma invasion: Choosing tools to meet the need

Cited by 12 publications

References 155 publications

Hydrogel on a Smart Nanomaterial Interface to Carry Therapeutics for Digitalized Glioma Treatment

Hydrogel on a Smart Nanomaterial Interface to Carry Therapeutics for Digitalized Glioma Treatment

Megalencephalic leukoencephalopathy with subcortical cysts 1 (MLC1) promotes glioblastoma cell invasion in the brain microenvironment

HepaCAM Suppresses Glioblastoma Stem Cell Invasion in the Brain

Contact Info

Product

Resources

About