Methods for growth of cultured cells in serum-free medium

Barnes, David W.; Sato, Gordon

doi:10.1016/0003-2697(80)90151-7

Cited by 954 publications

(296 citation statements)

References 29 publications

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“…However, a rational approach to the study of the hormonal control of proliferation and differentiation of cultured cells needs the partial or complete replacement, in the culture medium, of serum which is a complex, not yet fully characterized, mixture, variable in composition. Serum produces multiple uncontrollable hormonal interferences and thus may mask or affect the response of cells to purified hormones or factors (Barnes and Sato, 1980). Moreover, high serum medium does not reflect the physiological environment of the thyroid follicular cell in situ.…”

Section: Discussionmentioning

confidence: 99%

Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors

Roger¹,

Dumont²

1984

Molecular and Cellular Endocrinology

199

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Section: Discussionmentioning

confidence: 99%

Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors

Roger¹,

Dumont²

1984

Molecular and Cellular Endocrinology

199

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“…In fact, serum is believed to contribute to a phenomenon known as "culture shock," in which mammalian cells are exposed to an unrelenting onslaught of mitogenic signals that may lead to DNA damage, checkpoint activation, and senescence (for review, see Sherr and DePinho 2000). To overcome the pitfalls associated with culture shock, we adapted cell culture conditions similar to those described previously (Barnes and Sato 1980;Loo et al 1987), where serum was replaced with epidermal growth factor (EGF), insulin, high density lipoprotein (HDL), and transferrin (see Materials and Methods for more details).…”

Section: Mefs Grown In Serum-free Conditions Are Immortalmentioning

confidence: 99%

Activated oncogenes promote and cooperate with chromosomal instability for neoplastic transformation

Woo¹,

Poon²

2004

Genes Dev.

113

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Most cancer cells are aneuploid. The chromosomal instability hypothesis contends that aneuploidy is the catalyst for transformation, whereas the gene mutation hypothesis asserts that cancer is driven by mutations to proto-oncogenes and tumor-suppressor genes, with the aneuploidy a side effect of tumorigenesis. Because genotoxic stress induced by "culture shock" can obscure the transforming potential of exogenous genes, we cultured wild-type and p53 −/− mouse embryo fibroblasts in a more physiological (serum-free) environment. Under these conditions, the cells were immortal and, more importantly, chromosomally stable. Expression of oncogenic H-RasV12 did not induce senescence, but sensitized these cells to p53-dependent apoptosis. In addition, H-RasV12 induced chromosomal instability, as well as accumulation and phosphorylation of p53. Significantly, whereas cells grown under standard conditions could be transformed by coexpression of H-RasV12 and E1A, the chromosomally stable cells were refractory to transformation, as measured by anchorage-independent growth and tumor formation in nude mice. These oncogenes required a third genetic alteration that abolished the p53 pathway to create a permissive environment that promotes rapid chromosomal instability and transformation. Oncogene-induced chromosomal instability and transformation was attenuated by antioxidants. These data indicate that chromosomal instability could be a catalyst for oncogenic transformation, and bring together aspects of the chromosomal instability hypothesis and the gene mutation hypothesis for tumorigenesis.[Keywords: Chromosomal instability; oncogenic Ras; p53; transformation] Supplemental material is available at http://www.genesdev.org.

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“…These extracellular cues may be classified into several distinct groups, including growth factors, hormones, neuropeptides, morphogens, and nutrients such as amino acids and essential metals [1,2]. A challenge is to define the complete repertoire of extrinsic cues that are required for the growth of a specific cell or organ type.…”

Section: Introductionmentioning

confidence: 99%

Identification of iron-loaded ferritin as an essential mitogen for cell proliferation and postembryonic development in Drosophila

2010

Cell Res

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Animal cells require extrinsic cues for growth, proliferation and survival. The propagation of Drosophila imaginal disc cells in vitro, for example, requires the supplementation of fly extract, the composition of which remains largely undefined. Here I report the biochemical purification of iron-loaded ferritin as an active ingredient of fly extract that is required for promoting the growth of clone 8 imaginal disc cells. Consistent with an essential role for ironloaded ferritin in cultured cells, overexpression of ferritin or addition of iron in a nutrient-poor diet increases animal viability and body weight, promotes cell proliferation, and shortens the duration of postembryonic development. Conversely, overexpression of dominant-negative ferritin or addition of iron chelator causes the opposite effects. Ferritin mutant flies arrest development at the first-instar larval stage with a severe starvation phenotype reminiscent of that seen in starved larvae. I conclude that iron-loaded ferritin acts as an essential mitogen for cell proliferation and postembryonic development in Drosophila by maintaining iron homeostasis and antagonizing starvation response.

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Methods for growth of cultured cells in serum-free medium

Cited by 954 publications

References 29 publications

Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors

Factors controlling proliferation and differentiation of canine thyroid cells cultured in reduced serum conditions: effects of thyrotropin, cyclic AMP and growth factors

Activated oncogenes promote and cooperate with chromosomal instability for neoplastic transformation

Identification of iron-loaded ferritin as an essential mitogen for cell proliferation and postembryonic development in Drosophila

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