Methods for Enrichment of Bacterial Persister Populations for Phenotypic Screens and Genomic Studies

Adikari, Samantha; Hong-Geller, Elizabeth; Micheva-Viteva, Sofiya

doi:10.1007/978-1-0716-1621-5_5

Cited by 1 publication

(2 citation statements)

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“…If the purity of the sample cannot be improved, the high-throughput detection of gene expression cannot be achieved, such as RNA sequencing. Considering these challenges, a variety of enrichment methods have been applied to study the gene expression of the persistent phenotype [ 86 ]. The antibiotic lysis of sensitive bacteria is a conventional way of obtaining the remaining persister subpopulation [ 87 , 88 , 89 ].…”

Section: Recent Progress Of Technologies On Persister Studiesmentioning

confidence: 99%

“…Similarly, exposure to a stress environment, such as extreme temperature or nutrient restriction, and waiting for the aging of normal bacteria can induce persister survival [ 90 ]. Another method is flow sorting for persister cells via the fluorescent staining of bacterial cell membranes [ 86 ]. This method utilizes the intrinsic features in that the multidrug tolerance of persisters is correlated with depolarization, and that they express low proton motive force in the exponential division phase.…”

Section: Recent Progress Of Technologies On Persister Studiesmentioning

confidence: 99%

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Recent Advances in Bacterial Persistence Mechanisms

Pan,

Liu,

et al. 2023

IJMS

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The recurrence of bacterial infectious diseases is closely associated with bacterial persisters. This subpopulation of bacteria can escape antibiotic treatment by entering a metabolic status of low activity through various mechanisms, for example, biofilm, toxin–antitoxin modules, the stringent response, and the SOS response. Correspondingly, multiple new treatments are being developed. However, due to their spontaneous low abundance in populations and the lack of research on in vivo interactions between persisters and the host’s immune system, microfluidics, high-throughput sequencing, and microscopy techniques are combined innovatively to explore the mechanisms of persister formation and maintenance at the single-cell level. Here, we outline the main mechanisms of persister formation, and describe the cutting-edge technology for further research. Despite the significant progress regarding study techniques, some challenges remain to be tackled.

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