Methods for disruption of microbial cells for potential use in the dairy industry—a review

Geciova, J.; Bury, D.; Jelen, P.

doi:10.1016/s0958-6946(02)00038-9

Cited by 297 publications

(201 citation statements)

References 36 publications

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“…The cytolysis of fission yeast in pellet (D) was confi rmed in cells treated with HNO 3 at concentration of 36% or above. Various cell disruption strategies have been used to develop an efficient, low-cost, and effective method for release of intracellular products (Middelberg, 1995;Geciova et al, 2002;Ho et al, 2008;Klimek-Ochab et al, 2011;Bzducha-Wróbel et al, 2014). Unfortunately, the method of cell wall digestion reported in previous studies to might be too expensive.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast

et al. 2017

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-The distribution of metal and metalloid species in each of the cell compartments is termed as "metallome". It is important to elucidate the molecular mechanism underlying the beneficial or toxic effects exerted by a given metal or metalloid on human health. Therefore, we developed a method to measure intracellular metal ion concentration (particularly, intracellular calcium ion) in fission yeast. We evaluated the effects of nitric acid (HNO 3 ), zymolyase, and westase treatment on cytolysis in fission yeast. Moreover, we evaluated the changes in the intracellular calcium ion concentration in fission yeast in response to treatment with/without micafungin. The fission yeast undergoes lysis when treated with 60% HNO 3 , which is simpler and cheaper compared to the other treatments. Additionally, the intracellular calcium ion concentration in 60% HNO 3 -treated fission yeast was determined by inductively coupled plasma atomic emission spectrometry. This study yields significant information pertaining to measurement of the intracellular calcium ion concentration in fission yeast, which is useful for elucidating the physiological or pathological functions of calcium ion in the biological systems. This study is the first step to obtain perspective view on the effect of the metallome in biological systems.

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Section: Resultsmentioning

confidence: 99%

Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast

et al. 2017

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“…This method leads to a direct damage that is induced by the highspeed spinning of fine beads along with the biomass slurry as represented in (Fig. 6) [19,20]. Disruption depends mainly on the residence time of the beads in the system [21].…”

Section: Bead Millingmentioning

confidence: 99%

An Insight on Algal Cell Disruption for Biodiesel Production

Aarthy

Smita

Turkar

et al. 2018

Asian J Pharm Clin Res

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Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae. Result:The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used. Conclusion:In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

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“…Lipid-solvent systems are governed by the principle of likedissolves-like in which lipids are extracted using non-polar organic solvents, such as chloroform or hexane (Geciova et al, 2002). This extraction can be classified into five steps: (1) the microalgae are exposed to the solvents, which penetrate the cell membrane and enter the cytoplasm; (2) the solvent interacts with neutral lipids via van der Waal's forces to form a solvent-lipid complex; (3) this complex diffuses across the cell membrane, such that the neutral lipids enter the organic phase, while water and solvent-contaminant complexes (with carbohydrates or proteins) enter the aqueous phase; (4) the organic phase is further separated; and (5) crude lipids are transesterified to generate biodiesel (Lewis et al, 2000;Halim et al, 2012;Bahadar and Khan, 2013).…”

Section: Organic Solvent Extractionmentioning

confidence: 99%

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

Klinthong

Yang

Huang

et al. 2015

Aerosol Air Qual. Res.

209

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Fossil fuels, which are recognized as unsustainable sources of energy, are continuously consumed and decreased with increasing fuel demands. Microalgae have great potential as renewable fuel sources because they possess rapid growth rate and the ability to store high-quality lipids and carbohydrates inside their cells for biofuel production. Microalgae can be cultivated on opened or closed systems and require nutrients and CO 2 that may be supplied from wastewater and fossil fuel combustion. In addition, CO 2 capture via photosynthesis to directly fix carbon into microalgae has also attracted the attention of researchers. The conversion of CO 2 into chemical and fuel (energy) products without pollution via this approach is a promising way to not only reduce CO 2 emissions but also generate more economic value. The harvested microalgal biomass can be converted into biofuel products, such as biohydrogen, biodiesel, biomethanol, bioethanol, biobutanol and biohydrocarbons. Thus, microalgal cultivation can contribute to CO 2 fixation and can be a source of biofuels. This article reviews the literature on microalgae that were cultivated using captured CO 2 , technologies related to the production of biofuels from microalgae and the possible commercialization of microalgae-based biofuels to demonstrate the potential of microalgae. In this respect, a number of relevant topics are addressed: the nature of microalgae (e.g., species and composition); CO 2 capture via microalgae; the techniques for microalgal cultivation, harvesting and pretreatment; and the techniques for lipid extraction and biofuel production. The strategies for biofuel commercialization are proposed as well.

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Methods for disruption of microbial cells for potential use in the dairy industry—a review

Cited by 297 publications

References 36 publications

Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast

Evaluation of a novel method for measurement of intracellular calcium ion concentration in fission yeast

An Insight on Algal Cell Disruption for Biodiesel Production

A Review: Microalgae and Their Applications in CO2 Capture and Renewable Energy

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