Methods for Determination of Prekallikrein in Plasma, Glandular Kallikrein and Urokinase

Abstract

New chromogenic tripeptide substrates have been used for the determination of kallikreins and urokinase. The conditions have been optimized. It is possible to determine prekallikrein in plasma after activation with Cephotest®. No significant loss in activity caused by plasma kallikrein inhibitors is observed at the dilutions used.

Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus

“…4 ) The fibrinolytic activity of each enzyme (F-III-2, F-I1I-I, F-I1, F-I-2, F-I-I, and:F-I-O) was 204, 223, 22, 5.6, 6.1, and 3.0CU/mg, respeCtively using human plasmin 2 ' 1 ) as a standard. The enzymes also had strong ami do lytic activity 7) for various,chromogenic substrates as summarized in Table II. …”

Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins.

“…The enteric-coated gelatin capsule contained, in addition to the HMW-UK powder, 6 mg sodium chloride (Wako Pure Chemical Industries, Ltd., Japan), 24 2,4,6,8,10, and 24 h in the oral group, and at 0.5, 1, 2, 4, and 6 h in the intravenous group. 30 additional human subjects were separated into three groups.…”

Substrate specificity of tissue plasminogen activator and urokinase as determined with synthetic chromogenic substrates.

“…The differences in buffer did not give rise to any significant difference in substrate hydrolysis. The pH optimum of chromogenic substrate was broad (CLAESON et al, 1978). The plasminogen activator was dissolved in each of the buffer solutions.…”