Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms

Serafim, Luísa S.; Lemos, Paulo C.; Levantesi, Caterina; Tandoi, Valter; Santos, Helena; Reis, Maria A.M.

doi:10.1016/s0167-7012(02)00056-8

Cited by 145 publications

(109 citation statements)

References 79 publications

Supporting

Mentioning

102

Contrasting

Unclassified

Order By: Relevance

“…However, annotation of a putative acyl-CoA:DAG acyltransferases (AtfA), catalyzing the final step in the synthesis of triacylglycerols, indicates the potential for lipid storage (Kalscheuer and Steinbüchel, 2003). However, triacylglycerols are unlikely to accumulate in large amounts, given that such inclusions would probably stain positive with Nile Blue A (Serafim et al, 2002) and none were detected in the B45 phylotype (Speirs et al, 2009). …”

Section: Potential Storage Polymersmentioning

confidence: 99%

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

et al. 2016

View full text Add to dashboard Cite

Overgrowth of filamentous bacteria in activated sludge wastewater treatment plants (WWTPs) leads to impaired sludge settleability, a condition known as bulking, which is a common operational problem worldwide. Filaments with the Eikelboom 0092 morphotype are commonly associated with such bulking episodes. Members of the uncultured B45 phylotype, which is embraced within the phylum Chloroflexi, were recently shown to exhibit this morphology. Although these organisms are among the most abundant populations recorded in activated sludge processes, nothing is known about their metabolic characteristics. In this study, a genome sequence, representing the B45 phylotype, was retrieved from a metagenome generated from an activated sludge WWTP. The genome consisted of two chromosomes and one plasmid, which were 4.0, 1.0 and 0.04 Mbps in size, respectively. A metabolic model was constructed for this organism, based on annotation of its genome, showing its ability to generate energy by respiration, utilizing oxygen, nitrite or nitrous oxide as electron acceptors, or by fermentation of sugars. The ability of B45 members to ferment sugars under anaerobic conditions was validated in situ with microautoradiography-fluorescence in situ hybridization. The provisional name of 'Candidatus Promineofilum breve' is proposed for this species. This study represents the first detailed information on an uncultured genus of filamentous organisms from activated sludge.

show abstract

Section: Potential Storage Polymersmentioning

confidence: 99%

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The culture was grown at 28 °C, 150 rpm for 24 h on an orbital shaking incubator. The starter culture was used for 2) Accumulation/production of P(3HB), 160 ml of F4 mineral production medium (composed of (in g L -1 ) glucose, 40.0 g; urea, 3.0 g; citric acid, 0.2 g; sodium citrate, 4.0 g; and 1.0 ml trace element solution (MgSO 4 0.2 g; CaCl 2 , 0.01 g; Na 2 MoO 4 , 0.005 g; Z n SO 4 , 0.1 g; FeCl 3 , 0.05 g) was inoculated with 40 ml of the starter culture in 500 ml Erlenmeyer finned flasks and incubated at 28 °C, 200 rpm for 72 h.…”

Section: Production Of P(3hb)mentioning

confidence: 99%

“…controlled-release of drugs. 3,18 Through TGA analysis of the recovered P(3HB) from the DCC, the onset temperature ranged from 219-223 °C and the maximum degradation temperature varied from 270-292 °C. These values were higher than those for the polymers recovered from the FCC, the onset temperature ranged from 208-212 °C, and the maximum degradation temperature varied from 241-269 °C.…”

Section: Differential Scanning Calorimetry (Dsc) and Thermal Gravimetmentioning

confidence: 99%

“…Poly (3-hydroxybutyrate) (P(3HB)) is one of the most common PHAs and it is produced by a variety of organisms. [1][2][3] In bacteria, PHAs accumulate in the cytoplasm as insoluble polyester inclusion bodies that can represent up to 90% of the biomass. 4 PHAs are energy storage metabolites and are produced in response to stress, usually under nutrient limiting conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The extraction step is the most problematic and expensive, representing 50% or more of the total cost of production. 3 Furthermore, the extraction process directly affects the quality of the recovered polymer. Therefore, research has focused on improving and minimizing the costs associated with extraction.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simplified recovery process of Ralstonia solanacearum-synthesized polyhydroxyalkanoates via chemical extraction complemented by liquid-liquid phase separation

Macagnan¹,

Rodrigues²,

Alves³

et al. 2016

Quim. Nova

View full text Add to dashboard Cite

Poly (3-hydroxybutyrate) (P(3HB)) is the most studied thermoplastic biopolymer belonging to the polyhydroxyalkanoate (PHA) family, the main features of which include rapid biodegradability and biocompatibility. The bioplastic recovery process is an important step during production and can directly influence the characteristics of PHAs. However, more efficient methods for the production of P(3HB) are necessary to make it economically viable. The aim of the present study was to improve the standard, chloroformbased, extraction step for the recovery of P(3HB). The polymer was produced using a Ralstonia solanacearum strain. The following parameters were improved in the recovery process: heating time, separation method (filtration or liquid-liquid phase separation), biomass state (fresh or dry cell concentrate) and the solvent:biomass ratio. By improving the chemical extraction of P(3HB) we recovered 98% of the cumulative polymer and reduced the heating time by 75%. Furthermore, we improved the separation process and developed an extraction solution that was faster and more economical.

show abstract

References

2010

Wastewater Microbiology

View full text Add to dashboard Cite

Methods for detection and visualization of intracellular polymers stored by polyphosphate-accumulating microorganisms

Cited by 145 publications

References 79 publications

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

Genomic and in situ investigations of the novel uncultured Chloroflexi associated with 0092 morphotype filamentous bulking in activated sludge

Simplified recovery process of Ralstonia solanacearum-synthesized polyhydroxyalkanoates via chemical extraction complemented by liquid-liquid phase separation

References

Contact Info

Product

Resources

About