Methods for biosensing and imaging

Sensing Mechanism of Cysteine Specific Fluorescence Probes and Their Application of Cysteine Recognition

“…Cysteine fluorescent probes have emerged because of their useful properties, such as facilitating visibility, nondestructive imaging, and multilayer fluorescence imaging is a technique used in biological and biomedical research to visualize and study structures within cells or tissues using fluorescence microscopy. − Photoinduced Electron Transfer (PIET), Förster Resonance Energy Transfer (FRET), Internal Charge Transfer (ICT), phenomena observed in certain molecules where an electron is transferred from one part of the molecule to another, and Excited State Intramolecular Proton Transfer (ESIPT) , are only a few of the fluorescence emission mechanisms that have been used. ,, …”

“…In this scenario, the development of reliable methods for promptly and precisely identifying Cys both in laboratory settings and in living organisms holds significant significance within the realms of chemistry and the study of Cys biology. This encompasses research into Cys production, dispersion, specific concentrations, and metabolic pathways within biological entities. , To devise effective techniques for monitoring Cys, researchers typically consider several crucial factors: (1) Cys concentrations exhibit variability across diverse biological specimens, encompassing cells and biofluids. (2) The high reactivity of Cys underscores the importance of rapid in situ detection.…”