Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: A consensus report

Seed, Jennifer; Chapin, Robert E.; Clegg, Eric D.; Dostal, Lori A.; Foote, R.H.; Hurtt, Mark E.; Klinefelter, Gary R.; Makris, Susan L.; Perreault, Sally D.; Schrader, Steven M.; Seyler, David E.; Sprando, Robert L.; Treinen, Kimberley A.; Veeramachaneni, D. N. Rao; Wise, L. David

doi:10.1016/0890-6238(96)00028-7

Cited by 332 publications

(216 citation statements)

References 20 publications

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“…Sperm Motility Assays-Sperm from whole epididymis were obtained as described above from Harlan Sprague-Dawley rats (23). A drop of the sperm suspension was then diluted in warmed, modified Tyrode's albumin/lactate/pyruvate (TALP) solution (24) (114 mM NaCl, 3.2 mM KCl, 2 mM NaHCO 3 , 0.4 mM NaH 2 PO 4 H 2 O, 10 mM sodium lactate, 2 mM 1 The abbreviation used is: PBS, phosphate-buffered saline.…”

Section: Methodsmentioning

confidence: 99%

Sperm Motility Is Dependent on a Unique Isoform of the Na,K-ATPase

Woo

James

Lingrel³

2000

Journal of Biological Chemistry

144

162

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The Na,K-ATPase, a member of the P-type ATPases, is composed of two subunits, ␣ and ␤, and is responsible for translocating Na ؉ out of the cell and K ؉ into the cell using the energy of hydrolysis of one molecule of ATP. The electrochemical gradient it generates is necessary for many cellular functions, including establishment of the plasma membrane potential and transport of sugars and ions in and out of the cell. Families of isoforms for both the ␣ and ␤ subunits have been identified, and specific functional roles for individual isoforms are just beginning to emerge. The ␣4 isoform is the most recently identified Na,K-ATPase ␣ isoform, and its expression has been found only in testis. Here we show that expression of the ␣4 isoform in testis is localized to spermatozoa and that inhibition of this isoform alone eliminates sperm motility. These data describe for the first time a biological function for the ␣4 isoform of the Na,KATPase, revealing a critical role for this isoform in sperm motility.

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Section: Methodsmentioning

confidence: 99%

Sperm Motility Is Dependent on a Unique Isoform of the Na,K-ATPase

Woo

James

Lingrel³

2000

Journal of Biological Chemistry

144

162

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“…The percentage of motile sperm, i.e. sperm showing progressive movement, among 200 counted spermatozoa from each male is reported (SEED et al 1996).…”

Section: Epididymal Sperm Motilitymentioning

confidence: 99%

Sexual Maturation in Common Vole (<I>Microtus arvalis</I>) Males Raised under Laboratory Conditions

Miska

Kruczek

Kapusta

2014

folia biol (krakow)

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MISKA A., KRUCZEK M., KAPUSTA J. 2014. Sexual maturation in Common vole (Microtus arvalis) males raised under laboratory conditions. Folia Biologica (Kraków) 62: 135-142.The common vole is one of the most numerous rodents in Europe and Asia but its reproductive biology is not fully described. It is thought that females reach reproductive abilities at a very young age, however, there is no data concerning male sexual maturation. The aim of the present study was to determine the rate of sexual maturation of males of the common vole. Research was carried out on 4, 6, 8 and 10 week-old animals. Body, testes and accessory sex glands weights were compared to evaluate morphological development. Epididymal sperm quality was assessed by a motility test, sperm tail membrane integrity, viability, maturity and sperm head morphology. Moreover, the number of sperm cells in 1 mm ! of semen was evaluated. The largest body weights were observed in 8 and 10 week-old males, which also possessed the highest relative weights of gonads and accessory sex glands. These groups of males produce about 2 times more gametes than 6 week-old individuals. The highest seamen quality was noted in 8 and 10 week-old males. Based on the obtained results, it is concluded that among the investigated age groups the most appropriate male age for fertile copulation is between 8 and 10 weeks.

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“…After allowing 5 min for dispersion, a sperm aliquot was diluted 1:10 with fixative (10% formalin in PBS) and counted using a Neubauer chamber. Within 15 min, each uterine horn was injected with a volume containing 5 × 10 6 sperm [24]. One female was inseminated per male.…”

Section: Experiments 2 241 In Utero Insemination (Iui)mentioning

confidence: 99%

“…To analyze the sperm morphologically, smears were prepared on histological slides that were left to dry for 90 min and 200 spermatozoa per animal were analyzed in a phase-contrast microscope (400× magnification) [24]. Morphological abnormalities were classified into two general categories: head morphology (without curvature, without characteristic curvature, pin head or isolated form, i.e., no tail attached) and tail morphology (broken or rolled into a spiral) [25].…”

Section: Sperm Motility and Morphologymentioning

confidence: 99%