Methods and means of Stokes-polarimetry microscopy of optically anisotropic biological layers

Ушенко, А. Г.; Dubolazov, A. V.; Ушенко, В. А.; Ushenko, Yu. A.; Sakhnovskiy, M. Yu.; Sidor, M. I.; Prydiy, O. G.; Olar, O. I.; Lakusta, I. I.

doi:10.1117/12.2242628

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…The study was based on a two-component model for describing biological tissue structure: isotropic and anisotropic or crystalline. (Ushenko et al, 2016). The state of polarization of light changes when radiation passes through a sample of biological tissue and when reflected from its surface.…”

Section: Methodsmentioning

confidence: 99%

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Harazdiuk

Kozan

Kryvetskyi

et al. 2021

PMP

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Introduction: The article presents the current state of issues regarding the diagnosis of pathological conditions for forensic medical experts, in particular the study of normal muscle tissue and in the presence of hemorrhages. Particular attention is paid to biophysical research methods with evidence-based accuracy, objectivity, reproducibility, and rapid results. Objectives: The purpose of the research was to study the possibilities of using spectrophotopolyarimetric methods to analyze and determine the relationship of morphological structure of biological tissues with the temporal dynamics of changes in their optical parameters, to establish the possibility of differentiating necrotic changes and signs of damage by applying Mueller matrices of human muscle tissue samples. Results: During the study, we found a number of features and patterns of change in the properties of the laser beam as a result of passing through biological tissue. Polarization-correlation methods provide new, objective information about the dynamics of change in laser polarimetric imaging of morphological structure of biological tissues. By analyzing the statistical distributions of the ellipticity of polarization of laser images of normal human muscle tissue and in the presence of hemorrhages, the possibility of identifying the relationship between the development of statistically significant changes with increasing time of death and the possibility of differentiating them are considered. Conclusion: Based on these results, we can conclude that employing laser polarization methods will allow us to open new doors in the study of the biological tissues of the human body. The results showed the effectiveness of the studied methodology and the prospect of further research in this direction.

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Section: Methodsmentioning

confidence: 99%

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Harazdiuk

Kozan

Kryvetskyi

et al. 2021

PMP

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“…The large degree of variation within existing post mortem assessment methods prompts researchers to find new approaches to solve this problem. Ushenko et al (2016); Garazdyuk et al (2016); Vanchulyak et al (2019) have shown the possibilities of medico-physical methods of investigating the relationship between necrotic changes in the structure of biological tissues of different morphological structure, as well as causes and prescription of death. Purpose The purpose of the study was to develop an objectively reliable forensic methodology to enhance the functionality of the high-precision definition of TSD over a long period of time according to polarization microscopic tomography of the polycrystalline structure of the VB of the human eye by statistical and wavelet analysis.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Possibilities of Analysis of the Map of Linear Birefringence of the Crystal Fraction of Vitreous Body for Accurate Determination of the Time Since Death

Sarkisova

Bachynskіy

Palamar

et al. 2020

PMP

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Introduction: This paper discusses the possibility of polarization microscopic tomography of polycrystalline structure of vitreous body (VB) for use in forensics and in determining the time since death (TSD). Objectives: The purpose of this study was to develop a new set of forensic criteria to enhance the functionality of the high-precision definition of TSD over a long period of time according to polarization microscopic tomography of the polycrystalline structure of the VB of the human eye by statistical and wavelet analysis. Results: We obtained the numerical values of the change in the magnitude of the statistical moments of the 1-4 orders, which characterize the coordinate distributions of the magnitude of the linear birefringence (LB) of the polycrystalline component of the VB by the magnitude of the TSD. The results from our research illustrate the differences between the optical anisotropy of fibrillar collagen networks of VB layers with different TSD. The sensitivity range (36 hours) and accuracy (15 minutes) of the method of polarization tomography of the LB distributions of the polycrystalline component of the VB layers in the determined TSD were established. Conclusion: These results confirm the experimental processes outlined accurately determine the time of death. In turn, will provide scientific evidence, specifications thereof, and objective expert opinion.

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Computer Digital Differential Diagnostics of Aseptic and Septic Loosening of Endoprosthesis Cup of Artificial Hip Joint by Methods of Polarizing Microscopy

Vasyuk

Калашніков

Protsyuk³

et al. 2023

SpringerBriefs in Applied Sciences and Technology

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Methods and means of Stokes-polarimetry microscopy of optically anisotropic biological layers

Cited by 4 publications

References 23 publications

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Spectrophotopolarimetry as a Method for Determining the Time Dynamics of Changes in the Morphological Structure of Muscle Tissue

Diagnostic Possibilities of Analysis of the Map of Linear Birefringence of the Crystal Fraction of Vitreous Body for Accurate Determination of the Time Since Death

Computer Digital Differential Diagnostics of Aseptic and Septic Loosening of Endoprosthesis Cup of Artificial Hip Joint by Methods of Polarizing Microscopy

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