Methodology of Pulsed Photoacoustics and Its Application to Probe Photosystems and Receptors

Hou, Harvey J. M.; Sakmar, Thomas P.

doi:10.3390/s100605642

Cited by 13 publications

(7 citation statements)

References 93 publications

(119 reference statements)

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“…The evaluation of light energy storage (ES) was performed in accordance with the generally accepted principles and practical approaches 5 6 7 30 using the previously described fast Fourier transform (FFT) photoacoustic method 13 with modifications. Leaf discs (10 mm in diametre) were cut from the peripheral part of the corollas where the residual chlorophyll content is minimal.…”

Section: Methodsmentioning

confidence: 99%

“…Such an increase occurs only when the photobaric component is eliminated57. Considering the kinetic and spectral parametres of the PA-signal, a nonzero ES value provides an incontrovertible and direct evidence of either oxygenic or anoxygenic photosynthesis567.…”

mentioning

confidence: 99%

“…In this case switching on a strong background (non-modulated) light saturates the photochemistry, and the acoustic signal amplitude increases with a value equal to that of the energy storage (ES). Such an increase occurs only when the photobaric component is eliminated 5 7 . Considering the kinetic and spectral parametres of the PA-signal, a nonzero ES value provides an incontrovertible and direct evidence of either oxygenic or anoxygenic photosynthesis 5 6 7 .…”

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confidence: 99%

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Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Лысенко

Varduny

2013

Sci Rep

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Chlorophylless flower petals are known to be composed of non-photosynthetic tissues. Here, we show that the light energy storage that can be photoacoustically measured in flower petals of Petunia hybrida is approximately 10-12%. We found that the supposed chlorophylless photosynthesis is an anoxygenic, anthocyanin-dependent process occurring in blue flower petals (ADAPFP), accompanied by non-respiratory light-dependent oxygen uptake and a 1.5-fold photoinduced increase in ATP levels. Using a simple, adhesive tape stripping technique, we have obtained a backside image of an intact flower petal epidermis, revealing sword-shaped ingrowths connecting the cell wall and vacuole, which is of interest for the further study of possible vacuole-related photosynthesis. Approaches to the interpretations of ADAPFP are discussed, and we conclude that these results are not impossible in terms of the known photochemistry of anthocyanins.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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Anthocyanin-dependent anoxygenic photosynthesis in coloured flower petals?

Лысенко

Varduny

2013

Sci Rep

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“…Far-red light sources are widely used in photoacoustic studies of biochemical processes involving CET-PSI (Joliot et al, 2006;Hou and Sakmar, 2010). Besides, far-red light can be used in dualwavelength excitation experiments to test PSI and PSII coupling within a single linear electron transport chain (Lysenko, 2012).…”

Section: Introductionmentioning

confidence: 99%

Far-Red Spectrum of Second Emerson Effect: A Study Using Dual-Wavelength Pulse Amplitude Modulation Fluorometry

Лысенко¹,

Varduny²,

Симонович³

et al. 2014

American Journal of Biochemistry and Biotechnology

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Non-additive enhancement of the photosynthesis excited by simultaneous illumination with far-red light and light of shorter wavelengths is called as "second Emerson effect". Its action spectra are well-known as a photosynthetic yield's dependence on light wavelength in red (630-690 nm) spectral region at a constant-wavelength far-red illumination near 700-715 nm. However, the opposite dependence of the photosynthetic yield's of shorter constant-wavelength light (red or blue) on light wavelength in far-red (690-760 nm) spectral region was never studied. In this study the action spectrum of second Emerson effect was studied using a fast-Fourier dual-wavelength Pulse Amplitude Modulation (PAM) fluorometry. Chlorophyll fluorescence in ailanthus (Ailanthus altissima Mill.) leaves was excited with blue modulated light. Far-red induced decrease of fluorescence (fluorescence shift-FRIFS) was studied in response to illumination of leaves with a background light from 690 to 760 nm (10 nm step), calculating FRIFS = (F 0 -Fs)/F 0 , where F 0 -fluorescence measured without and Fs-with far-red light. Maximum FRIFS was observed at 720 nm (11.8%), but it still remained considerable at 740, 750 nm and a low FRIFS values were revealed at 690 and even at 760 nm. Measurements carried out with blue saturating flashes during and after far-red illumination showed the increase of quantum yield of Photosystem II (PSII), calculated as Fv/Fm at 720 nm background light. FRIFS had lower values under excitation with red modulating light. It is concluded that FRIFS is a result of a photochemical quenching caused by an additional selective far-red excitation of PSI in conditions when PSII is preferably excited by blue light thus leading the PSI to limit non-cyclic electron flow. The contradiction between the known absorption spectra of PSI-light harvesting complex I and the observed action spectrum of second Emerson effect (FRIFS spectrum) is discussed.

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“…Pulsed photoacoustic spectroscopy can provide a direct measurement of thermodynamic parameters such as volume change and enthalpy changes that accompany electron transfer reactions (Braslavsky, 1985;Carpentier et al, 1990;Arnaut et al, 1992;Small et al, 1992;Losi et al, 1995;Edens et al, 2000;Malkin, 2000;Herbert et al, 2001;Feitelson and Mauzerall, 2002;Delosme, 2003;Hou and Mauzerall, 2006;Hou and Sakmar, 2010;Hou and Mauzerall, 2011). With prior knowledge of the change in Gibbs free energy of the corresponding reactions, the apparent entropy change (TS) of the reaction can be calculated.…”

Section: Thermodynamics Of Mena and Menb Null Mutantsmentioning

confidence: 99%