Methodology of mycobacteria tuberculosis bacteria detection by Raman spectroscopy

Zyubin, Andrey; Lavrova, Anastasia I.; Manicheva, Olga; Dogonadze, Marine; Tsibul’nikova, A. V.; Самусев, И. Г.

doi:10.1117/12.2282967

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…We revealed spectral differences in deactivated strains of Beijing species for pulmonary clinical XDR and reference H37Rv strains [23]. Figure 1 shows the original spectra of MbT strains with different antibiotic susceptibility: sensitive (SENS), MDR, XDR.…”

Section: Spectral Differences In Clinical Pulmonary Strainsmentioning

confidence: 99%

Raman spectroscopy revealsM. tuberculosisstrains with different antibiotic susceptibility

et al. 2019

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The drug resistance of Mycobacterium tuberculosis (MbT) remains a serious challenge to global public health as extensively drug resistant tuberculosis usually leads to a lethal outcome. In the case of diseases with resistant strains, it is extremely important to identify a specific strain as soon as possible in order to start an adequate therapy. One such diagnostic method could be the Raman spectroscopy method. We investigated M. tuberculosis strains of the Beijing family deactivated by heating, with different drug sensitivity: sensitive, multi- and extensively drug resistant. Samples, obtained from patient specimens were investigated by Raman spectrometry with the He–Ne (632.8 nm) laser excitation source. As a result, a set of optimal experimental parameters for strain discrimination were determined based on the cell-wall spectral differences. These differences distinguish between drug-sensitive and drug-resistant strains as well as between strains isolated from material samples taken from patients suffering from pulmonary and extra-pulmonary forms of tuberculosis. We suggest that the obtained results allow the usage of the Raman spectroscopy as a fast diagnostic tool for determining various strains in clinical medical practice.

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Section: Spectral Differences In Clinical Pulmonary Strainsmentioning

confidence: 99%

Raman spectroscopy revealsM. tuberculosisstrains with different antibiotic susceptibility

et al. 2019

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“…In the latter case, the strain is subcultured on a Löwenstein-Jensen medium and the subculture (second generation) is stored in the collection. Detailed methodology of the suitable medium selection has been described previously [20]. We have investigated MbT clinical strains belonging only to the Beijing family to reduce interlinear variability.…”

Section: Microbial Preparationmentioning

confidence: 99%

The cell-wall structure variation in Mycobacterium tuberculosis with different drug sensitivity using Raman spectroscopy in the high-wavenumber region

Zyubin¹,

Lavrova²,

Manicheva³

et al. 2020

Laser Phys. Lett.

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Over the past decade Raman spectroscopy has been successfully used to study the cell structure of various pathogens, including M. tuberculosis (MbT). It is especially important due to the high ability of pathogen to become resistant to anti-tuberculosis drugs usually leading to a lethal outcome. In the present study we investigate deactivated MbT strains with different drug sensitivity (extra-, multi-and sensitive drug resistance) of the Beijing family. Samples have been obtained from patients suffering from pulmonary and extra-pulmonary forms of tuberculosis. Samples have been investigated by Raman spectrometry using a diode-pumped solid-state argon (514 nm) laser excitation source in the high-wavenumber region. As a result, protein and lipid vibrational bands for strain discrimination with various drug resistances have been determined. The obtained results can be used as fast diagnostic tool development for screening MbT strains in clinical medical practice.

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“…Vol. 10456. International Society for Optics and Photonics, 2018 [1] . Value of the data Raman spectra can be used for bacterial identification in vitro .…”

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confidence: 99%

Dataset of single Mycobacterium tuberculosis bacteria cells with different antibiotic susceptibility obtained by Raman spectroscopy

et al. 2018

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This data article contains Raman experimental data, obtained with Horiba Jobin-Yvon LabRam HR800 spectrometer (Japan), which can be used for rapid identification of Mycobacterium tuberculosis (MbT) bacteria (Beijing clade) in vitro. Data present analyzed Raman spectra of bacterial cells with various drug resistances obtained from pulmonary and extra pulmonary samples. Data can provide information about characteristic maxima of different structures in biological cell.

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Methodology of mycobacteria tuberculosis bacteria detection by Raman spectroscopy

Cited by 4 publications

References 18 publications

Raman spectroscopy revealsM. tuberculosisstrains with different antibiotic susceptibility

Raman spectroscopy revealsM. tuberculosisstrains with different antibiotic susceptibility

The cell-wall structure variation in Mycobacterium tuberculosis with different drug sensitivity using Raman spectroscopy in the high-wavenumber region

Dataset of single Mycobacterium tuberculosis bacteria cells with different antibiotic susceptibility obtained by Raman spectroscopy

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