Methodology for soil respirometric assays: Step by step and guidelines to measure fluxes of trace gases using microcosms

Pitombo, Leonardo Machado; Ramos, Juliana C.; Quevedo, Helio Danilo; Carmo, Karina P. do; Paiva, Jane Maria Faulstich de; Pereira, Elisabete Alves; Carmo, Janaína Braga do

doi:10.1016/j.mex.2018.06.008

Cited by 11 publications

(10 citation statements)

References 24 publications

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“…The N 2 O fluxes were measured after the first 24 h (at 45% of WHC) and then at 1, 7, 15, and 30 days following wetting (at 70% of WHC). For each flux measurement, five gas samples were taken from the microcosm headspace using syringes at 1, 30, 60, and 90 min after closure of the microcosm, followed by the respirometric assay proposed by Pitombo et al (2018). The N 2 O concentrations were determined by gas chromatography, and the fluxes were calculated as described above.…”

Section: Soil Leaf Litter and Crop Residue Incubation Experimentsmentioning

confidence: 99%

Differences in N2O Fluxes and Denitrification Gene Abundance in the Wet and Dry Seasons Through Soil and Plant Residue Characteristics of Tropical Tree Crops

Nishisaka

Youngerman

Meredith

et al. 2019

Front. Environ. Sci.

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The conversion of forest to agricultural soils is a widespread activity in tropical systems, and its link to nitrous oxide (N 2 O) fluxes and nitrogen cycling gene abundance is relevant to understand environmental drivers that may interact with climate change. A current challenge to estimating N 2 O emissions from land use conversion is an incomplete understanding of crop-specific impacts on denitrifier communities and the N 2 O fluxes driven by differences in the above-and below-ground inputs with crop type. To address this knowledge gap in tree crops, we evaluated N 2 O fluxes and denitrification gene abundance and their relationships with soil and plant residue characteristics in citrus and eucalyptus plantations in the field and in soil incubations. We found that the accumulated N 2 O fluxes from soil were lower for the two agricultural field sites than those for their adjacent forest sites in dry and wet seasons. The N 2 O fluxes were higher in the wet season, and this seasonal difference persisted even when the soils collected from both seasons were incubated under the same moisture and temperature conditions in the lab for 30 days. Increased N 2 O fluxes in the wet season were accompanied by an increase in soil nirK and nosZ gene abundance, the dissolved organic carbon (DOC) concentration, and the total soil carbon (C) and nitrogen (N) content. In turn, the abundance of denitrifiers, as indicated by nirK, nirS, and nosZ gene copy numbers, showed a low but significant positive correlation with soil bulk density. Our results suggest that soil moisture, leaf litter, and crop residues influence the seasonal differences in both N 2 O fluxes and abundance of denitrifiers in citrus-and eucalyptus-cultivated soils, likely through effects on soil physicochemical characteristics. These findings highlight the overwhelming role of environmental drivers that can make investigating microbial drivers difficult in the field and open the possibility for a better understanding of N cycling processes in tropical soils based on paired field-and incubation-based experimentation.

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Section: Soil Leaf Litter and Crop Residue Incubation Experimentsmentioning

confidence: 99%

Differences in N2O Fluxes and Denitrification Gene Abundance in the Wet and Dry Seasons Through Soil and Plant Residue Characteristics of Tropical Tree Crops

Nishisaka

Youngerman

Meredith

et al. 2019

Front. Environ. Sci.

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“…One approach is to use isolated components of oil in experiments that utilize principles of mineralization i.e. oil biodegradation is derived from production of respired carbon dioxide or other gases (Phillips and Nickerson 2015;Pitombo et al 2018). In hydrocarbon biodegradation studies, these respirometry assays typically utilize radiolabeled substrates and are commonly referred to as mineralization or mineralization assays (Steven et al 2007;Aislabie et al 2012).…”

Section: Quantifying Oil Biodegradationmentioning

confidence: 99%

Hydrocarbon biodegradation potential of microbial communities from high Arctic beaches in Canada's Northwest Passage

Ellis

Altshuler

Schreiber

et al. 2022

Marine Pollution Bulletin

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“…The release of electrons from organic matter degradation in the anode chamber results in the production of several gaseous products, most notably CO 2 and CH 4 , but also trace levels of N 2 O, NO, N 2 , H 2 S, and H 2 (Pitombo et al 2018).…”

Section: Effects Of Potassium Ferricyanide Concentration On Total Gas...mentioning

confidence: 99%

Electrical generation and methane emission from an anoxic riverine sediment slurry treated by a two-chamber microbial fuel cell

Xiao

Yang

et al. 2022

Environ Sci Pollut Res

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A two-chamber slurry microbial fuel cell (SMFC) was constructed using black-odorous river sediments as substrate for the anode. We tested addition of potassium ferricyanide (K 3 [Fe(CN) 6 ]) or sodium chloride (NaCl) to the cathode chamber (0, 50, 100, 150, and 200 mM) and aeration of the cathode chamber (0, 2, 4, 6, and 8 h per day) to assess their response on electrical generation, internal resistance, and methane emission over a 600-h period. When the aeration time in the cathode chamber was 6 h and K 3 [Fe(CN) 6 ] or NaCl concentrations were 200 mM, the highest power densities were 6.00, 6.45, and 6.64 mW•m −2 , respectively. With increasing K 3 [Fe(CN) 6 ] or NaCl concentration in the cathode chamber, methane emission progressively decreased (mean ± SD: 181.6 ± 10.9 → 75.5 ± 9.8 mg/m 3 •h and 428.0 ± 28.5 → 157.0 ± 35.7 mg/m 3 •h), respectively, but was higher than the reference having no cathode/anode electrodes (~ 30 mg/m 3 •h). Cathode aeration (0 → 8 h/day) demonstrated a reduction in methane emission from the anode chamber for only the 6-h treatment (mean: 349.6 ± 37.4 versus 299.4 ± 34.7 mg/m 3 •h for 6 h/day treatment); methane emission from the reference was much lower (85.3 ± 26.1 mg/m 3 •h). Our results demonstrate that adding an electron acceptor (K 3 [Fe(CN) 6 ]), electrolyte solution (NaCl), and aeration to the cathode chamber can appreciably improve electrical generation efficiency from the MFC. Notably, electrical generation stimulates methane emission, but methane emission decreases at higher power densities.

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Methodology for soil respirometric assays: Step by step and guidelines to measure fluxes of trace gases using microcosms

Abstract: Graphical abstract

Cited by 11 publications

References 24 publications

Differences in N2O Fluxes and Denitrification Gene Abundance in the Wet and Dry Seasons Through Soil and Plant Residue Characteristics of Tropical Tree Crops

Differences in N2O Fluxes and Denitrification Gene Abundance in the Wet and Dry Seasons Through Soil and Plant Residue Characteristics of Tropical Tree Crops

Hydrocarbon biodegradation potential of microbial communities from high Arctic beaches in Canada's Northwest Passage

Electrical generation and methane emission from an anoxic riverine sediment slurry treated by a two-chamber microbial fuel cell

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