Methodology for meat species identification: A review

Hitchcock, C. H. S.; Crimes, Andrew A.

doi:10.1016/0309-1740(85)90077-4

Cited by 29 publications

(13 citation statements)

References 23 publications

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“…Meat speciation based on specific recognition through the antibody-antigen affinity has been applied for a long time (46) and several commercial kits have been developed for the determination of raw and moderately cooked meat in food. In 1995, Hofmann et al reported on the application of a commercial ELISA kit for the analysis of feed samples, demonstrating that MBM cannot be detected in feed that has been previously sterilised at elevated temperatures (above 120°C) (48).…”

Section: Immunochemical Methodsmentioning

confidence: 99%

An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy

Gizzi

Raamsdonk²,

Baeten³

et al. 2003

Rev. Sci. Tech. OIE

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Section: Immunochemical Methodsmentioning

confidence: 99%

An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy

Gizzi

Raamsdonk²,

Baeten³

et al. 2003

Rev. Sci. Tech. OIE

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“…Numerous analytical methodologies have been developed for this purpose such as physicochemical, electrophoretic, chromatographic, immunological [5,6]; however, none of these conventional approaches have been conclusively proved for authentic identification due to one or the other inherent limitation. On the other hand, owing to their repeatability and reproducibility, the DNA-based molecular techniques have promisingly emerged in the recent past [7].…”

Section: Introductionmentioning

confidence: 99%

Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration

Karabasanavar¹,

Singh

Kumar³

et al. 2012

Eur Food Res Technol

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In order to prevent fraud in the sale and strengthen quality assurance, authentic identification of chicken meat is essential. In the present investigation, a chicken (Gallus gallus)-specific polymerase chain reaction (PCR) was developed for the unambiguous identification of chicken meat. The PCR assay employs pair of primers designed against chicken nuclear 5-aminolevulinate (ALA) synthase gene. Highly chicken-specific diagnostic amplicon of 288 bp was established upon PCR and was evident in all the nine breeds/strains of chicken species. Sensitivity of PCR in detecting chicken meat adulteration was established to be at 0.1 % in the foreign meat matrix, while limit of detection (LOD) of chicken DNA was 10 pg. Suitability of the developed chicken-specific PCR was validated and confirmed in raw, cooked/heat treated (60, 80, 100, and 121°C), and micro-oven cooked meat samples. Possibility of cross-amplification of adulterating DNA was excluded by cross-checking the developed PCR assay with several animal and avian species. The PCR assay developed in this study is highly promising for applications involving circumstances that require authentic identification of chicken meat.

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“…Η πιο κοινή προσέγγιση για την ανάλυση αυτών των πρωτεϊνών είναι η ανάπτυξη ανοσοδοκιμών, κάνοντας χρήση της εξειδικευμένης αντίδρασης αντισώματος-αντιγόνου (Hitchcock & Crimes, 1985). Μολονότι η μέθοδος Enzyme-Linked Immuno-absorbent Assay (ELISA) έχει ευρείες εφαρμογές στην ανάλυση των τροφίμων και των ζωοτροφών, είναι σημαντικό να αντιληφθεί κανείς ότι η θετική αντίδραση που αφορά στην παρουσία ειδικών PAPs που λαμβάνονται με μια ανοσοδοκιμή έχει κυρίως ενδεικτικό μόνο χαρα κτήρα και είναι ανάγκη να επιβεβαιωθεί με άλλες μεθόδους.…”

Section: μέθοδοι ανοσοδοκιμής (Immunoassays) και ανί χνευσης Dnaunclassified

Feed Ban and BSE: the detection and identification of processed animal proteins in compound feeds

Zoiopoulos¹

2017

J Hellenic Vet Med Soc

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Reference is made initially to the incidence of BSE within the EU territory and the reasons of the appearance of the disease i.e. the use of meat and bone meal in ruminant diets. In turn, the evolution of Community legislation in the sector of marketing of feeds of animal origin is described. Furthermore, the banning of feeding of mammalian protein to ruminants and the extension of prohibition to further animal proteins such as from other vertebrates and how this banning affects the feeding of the various animal species is reviewed. Finally, the importance of detecting and identifying the presence and amount of processed animal proteins in compound feed as well as feed materials is stressed and methods such as feed microscopy, immunoassay techniques, PCR, NIR and HPLC are discussed.

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Methodology for meat species identification: A review

Cited by 29 publications

References 23 publications

An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy

An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy

Development and application of highly specific PCR for detection of chicken (Gallus gallus) meat adulteration

Feed Ban and BSE: the detection and identification of processed animal proteins in compound feeds

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