1997
DOI: 10.1016/s0887-2333(97)00002-7
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Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture

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Cited by 210 publications
(228 citation statements)
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“…Previously, using the alamar Blue and CFDA-AM cytotoxicity assays, we had observed that cytotoxicity was more pronounced 24 h after the termination of a concurrent fluoranthene and UV exposure than after 2 h. In the case of fluoranthene photocytotoxicity, the membrane impairment appears to be due to the generation of reactive oxygen species (ROS) within the membrane (Schirmer et al, 1997). In the case of direct cytotoxicity, the membrane impairment appears to be due to a different mechanism, likely the physical disruption of membrane integrity and fluidity by the presence of PAH molecules.…”
Section: Discussionmentioning
confidence: 99%
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“…Previously, using the alamar Blue and CFDA-AM cytotoxicity assays, we had observed that cytotoxicity was more pronounced 24 h after the termination of a concurrent fluoranthene and UV exposure than after 2 h. In the case of fluoranthene photocytotoxicity, the membrane impairment appears to be due to the generation of reactive oxygen species (ROS) within the membrane (Schirmer et al, 1997). In the case of direct cytotoxicity, the membrane impairment appears to be due to a different mechanism, likely the physical disruption of membrane integrity and fluidity by the presence of PAH molecules.…”
Section: Discussionmentioning
confidence: 99%
“…At confluency, the culture medium was removed and each well rinsed once with 500 vl of L-15/ex, a modification of Leibovitz's L-15 medium developed specifically for presenting PAHs to the cells either in the dark or with a concurrent UV radiation exposure (Schirmer et al, 1997). A description of the constituents of L-15/ex and preparation procedures have been outlined previously (Schirmer et al, 1997). After the rinse, all wells received 500 vl of L-15/ex.…”
Section: Cell Culture and Cytotoxicity Testingmentioning
confidence: 99%
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“…The fluorescent dyes alamarBlue™ (BioSource, Solingen, Germany) and 5-carboxyfluorescein diacetate acetoxymethyl ester (CFDA-AM, Molecular Probes, Eugene, OR, USA) were used in combination, as previously described, using L-15/ex as a simplified culture medium [27]. The alamarBlue™ dye measures the redox potential of a cell, and 5-carboxyfluorescein diacetate acetoxymethyl ester measures cell membrane integrity.…”
Section: Alamarblue™ and Cfda-am Testmentioning
confidence: 99%