Methodology Characterization of a rice germin-like protein gene promoter

Mahmood, Tariq; Yasmin, Tayyaba; Haque, Md. Intesaful; Naqvi, Syed Muhammad Saqlan

doi:10.4238/2013.february.7.6

Cited by 18 publications

(22 citation statements)

References 35 publications

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“…The wound inducibility of OsRGLP2 promoter is already known (Mahmood et al 2013); the novelty of the present study is that this promoter is functional in different host systems, in this case against fungal infection. The present study has illustrated that the OsRGLP2 promoter has strong local and systemic activity to F. solani and A. solani infection, and this response was greater than simple mechanical wounding.…”

Section: Discussionmentioning

confidence: 98%

“…These regulatory regions may have a role in response to water and salt stress, plant growth hormones, light sensitivity, storage proteins in seeds, and biotic stress. Further, recently Mahmood et al (2013)reported that the woundinducible property of this promoter in transgenic tobacco plants provides valuable insight for generating transgenic crop plants with enhanced defense-related features. Moreover, tissue specific activity of OsRGLP2 promoter was detected in the outer and inner phloem region of midrib, in the cortical area next to the phloem, in the epidermal cells, and in the veins of petals.…”

Section: Introductionmentioning

confidence: 99%

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Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Munir

Hayashi

Batley

et al. 2015

Funct Integr Genomics

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Controlled transgene expression via a promoter is particularly triggered in response to pathogen infiltration. This is significant for eliciting disease-resistant features in crops through genetic engineering. The germins and germin-like proteins (GLPs) are known to be associated with plant and developmental stages. The 1107-bp Oryza sativa root GLP2 (OsRGLP2) gene promoter fused to a β-glucuronidase (GUS) reporter gene was transformed into potato plants through an Agrobacterium-mediated transformation. The OsRGLP2 promoter was activated in response to Fusarium solani (Mart.) Sacc. and Alternaria solani Sorauer. Quantitative real-time PCR results revealed 4-5-fold increase in promoter activity every 24 h following infection. There was a 15-fold increase in OsRGLP2 promoter activity after 72 h of F. solani (Mart.) Sacc. treatment and a 12-fold increase observed with A. solani Sorauer. Our results confirmed that the OsRGLP2 promoter activity was enhanced under fungal stress. Furthermore, a hyperaccumulation of H2O2 in transgenic plants is a clear signal for the involvement of OsRGLP2 promoter region in the activation of specific genes in the potato genome involved in H2O2-mediated defense response. The OsRGLP2 promoter evidently harbors copies of GT-I and Dof transcription factors (AAAG) that act in response to elicitors generated in the wake of pathogen infection.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Munir

Hayashi

Batley

et al. 2015

Funct Integr Genomics

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“…It is explained that, under these conditions, PI genes are activated and JA is known to be a potent inducer of PIs. The OsRGLP2 promoter contains several putative stressresponsive regulatory regions (Mahmood et al, 2013;Munir et al, 2015), which might play significant roles in upregulating the stress-responsive genes including the PI-II genes.…”

Section: Molecular Analysismentioning

confidence: 99%

“…The PCAMBIA1391Z vector having the Solanum lycopersicum proteinase inhibitor-II gene (Accession No. AY007240) fused with the GUS under the control of the OsRGLP2 promoter (Mahmood et al, 2013; Figure 1) in Agrobacterium strain EHA101 was used to produce transgenic plants using the leaf disk method (Horsch et al, 1985).…”

Section: Plant Materials and Transformation Vectormentioning

confidence: 99%

“…Many stress-related genes have been expressed in transgenic plants using either constitutive or stressinducible promoters. The OsRGLP2 promoter (plantderived wound-inducible promoter) is a robust promoter capable of driving the expression of downstream genes under certain stress conditions, including wounding, salt, dehydration, and pathogenic infection (Mahmood et al, 2013;Munir et al, 2015). Therefore, expression of the PI-II gene under the OsRGLP2 promoter was investigated in salt-stressed transgenic Nicotiana benthamiana.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Rehman¹,

Jørgensen²,

Rasmussen³

et al. 2017

Turk J Biol

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Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better growth, higher chlorophyll content, higher proline contents, and higher average fresh weight when compared to the wild type. This comparative analysis of transgenic and wild-type seedlings allows us to suggest that PI-II gene activity can be upregulated via the OsRGLP2 promoter under osmotic stress conditions.

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Potential stress tolerance roles of barley germins and GLPs

Karlık

2021

Dev Genes Evol

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Methodology Characterization of a rice germin-like protein gene promoter

Cited by 18 publications

References 35 publications

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Germin-like protein 2 gene promoter from rice is responsive to fungal pathogens in transgenic potato plants

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Potential stress tolerance roles of barley germins and GLPs

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