Methodology A new strategy employed for identification of sweet orange cultivars with RAPD markers

Sun, Xin; Mu, Qian; Jiang, Ding; Wang, C.; Wang, X.C.; Fang, Jiapeng

doi:10.4238/2012.august.6.11

Cited by 11 publications

(6 citation statements)

References 17 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, this technique is regarded as one of the most intuitive forms of DNA fingerprinting application. As previously mentioned, CIDs have been successfully used in several fruit crop cultivars, such as sweet orange, pear, grapevine, loquat, and apple (Lin et al, 2011;Zhang et al, 2011;Zhao et al, 2011;Sun et al, 2012;Wang et al, 2012). In this study, the CID approach was first adopted to identify tomato cultivars, and then the entire appraisal process was recorded by CID, which provides information pertaining to the polymorphic primer and specific bands that were used for identification ( Figure 2).…”

Section: Discussionmentioning

confidence: 99%

“…Cultivar identification diagrams (CIDs) by RAPD were developed in the last 3years, and it has so far been used for the identification of sweet orange, pear, grapevine, loquat, and apple cultivars by Fang's group (Lin et al, 2011;Zhang et al, 2011;Zhao et al, 2011;Sun et al, 2012;Wang et al, 2012). As a novel strategy for cultivar identification using RAPD markers, CID overcomes the defects of the traditional analytic method, requiring fewer primers for RAPD-polymerase chain reaction (PCR) amplification, and the results are output in diagram form.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Cao

Zhou

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed. As verification of the CID validity, 8 randomly selected cultivars were investigated and proven to be well distinguished. In addition, 33 DNA bands were obtained, 20 (60.6%) of which were polymorphic. Genetic distances were calculated with a range of 0.032 to 1.402. Clustering analysis showed that the 64 tomato cultivars were divided into 4 groups with a similarity coefficient of 0.40. Using this novel strategy, with the same RAPD data, both CID and clustering analysis can simultaneously determine tomato cultivars and their genetic diversity.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Cao

Zhou

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Among several molecular markers available, random amplified polymorphic DNA (RAPD) is the simplest method (William et al 1990). RAPD has been widely used in determination of intraspecific as well as interspecific variations (Pharmawati et al 2004;Noormohammadi et al 2012), plant cultivar and varietal identification (Chaudhary et al 2010;Sun et al 2012). RAPD marker was proved to be able to identify hybridity of putative hybrids and their parents both in natural or spontaneous hybridizations (Tovae-Sanchez & Oyama 2004;El-Kamali et al 2010;Clarkson et al 2011;Yulita & Mansur 2012) as well as in artificial crosses (Chen & Mii 2012;Narendrula & Nkongolo 2012).…”

Section: Introductionmentioning

confidence: 97%

The Genetic Relationships of Grevillea Hybrids Determined by RAPD Marker

Pharmawati

Macfarlane

2013

HAYATI Journal of Biosciences

View full text Add to dashboard Cite

Grevillea (Proteaceae) is native to Australia and is known as garden plant. The flowers have high diversity colors, which range over orange, yellow, pink, red and green. It is a large genus, consisted of 357 species. This genus is economically important and there are hundreds of Grevillea hybrids with beautiful color and shape of flower. Information of the genetic relationships of Grevillea hybrids is not yet available. Ten Grevillea hybrids were used in this study to evaluate the genetic relationships between hybrids and with their parents. PCR-RAPD technique was employed in this study with seven RAPD primers. The dendrogram of Grevillea hybrids was performed using Neighbor-Joining analysis based on genetic distance. The analysis revealed that 'Moonlight', 'Caloundra Gem', and Little Pink Willie' were clustered with Grevillea banksii. The three hybrids were hybrid of G. banksii and other Grevillea species. 'Superb' was closely related to 'Robyn Gordon' as they are hybrid of G. banksii and G. bipinnatifida.

show abstract

“…DNA markers are a category of powerful tools used to identify plant cultivars and species, but there is no strong link between marker information and concrete plant varieties, and these markers have not yet been efficient in the identification of fruit crop varieties Sun et al, 2012). On the basis of these characteristics, Zhao et al (2011) came up with a novel strategy for the efficient identification of plant individuals relying on a new way of recording DNA fingerprints of genotyped plants, whereby a manual cultivar identification diagram (MCID) can be generated and used as a source of referable information for quick identification of plant and/or seed samples even in the future (Korir et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy

Chen

2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Manual cultivar identification diagram is a new strategy for plant cultivar identification based on DNA markers, providing information to efficiently separate cultivars. We tested 25 pairs of apple EST-SSR primers for amplification of PCR products from loquat cultivars. These EST-SSR primers provided clear amplification products from the loquat cultivars, with a relatively high transferability rate of 84% to loquat; 11 pairs of primers amplified polymorphic products. After analysis of 24 red-fleshed loquat accessions, we found that only 7 pairs of primers could clearly separate all of them. A cultivar identification diagram of the 24 cultivars was constructed using polymorphic bands from the DNA fingerprints and EST-SSR primers. Any two of the 24 cultivars could be rapidly separated from each other, according to the polymorphic bands from the cultivars; the corresponding primers were marked in the correct position on the cultivar identification diagram. This red-flesh loquat cultivar identification diagram can separate the 24 Red-flesh loquat cultivars and identification diagram red-flesh loquat cultivars, which is of benefit for loquat cultivar identification for germplasm management and breeding programs.

show abstract

Methodology A new strategy employed for identification of sweet orange cultivars with RAPD markers

Cited by 11 publications

References 17 publications

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

The Genetic Relationships of Grevillea Hybrids Determined by RAPD Marker

Rapid identification of red-flesh loquat cultivars using EST-SSR markers based on manual cultivar identification diagram strategy

Contact Info

Product

Resources

About