Methodologies for in Vitro Cultivation of Arbuscular Mycorrhizal Fungi with Root Organs

Cranenbrouck, Sylvie; Voets, Liesbeth; Bivort, Céline; Renard, L.; Strullu, D. G.; Declerck, Stéphane

doi:10.1007/3-540-27331-x_18

Cited by 83 publications

(63 citation statements)

References 41 publications

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“…This AMF was grown in association with Ri T-DNA transformed carrot (Daucus carota L.) roots clone DC1 (approximately 70 mm length) on Petri plates (90 mm diameter) containing the modified Strullu-Romand (MSR) medium, 38 supplemented with 10 g L ¡1 sucrose, 3 g L ¡1 phytagel and adjusted to pH 5.5. 39 The Petri plates were incubated for 3 months in the dark in an inverted position at 27 C. Several thousand spores were produced during this period.…”

Section: Biological Materialsmentioning

confidence: 99%

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

Velivelli

Lojan

Cranenbrouck

et al. 2015

Plant Signaling & Behavior

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Section: Biological Materialsmentioning

confidence: 99%

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

Velivelli

Lojan

Cranenbrouck

et al. 2015

Plant Signaling & Behavior

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“…The spores were extracted by solubilisation of the gellan gel (Doner and Becard 1991). An approximate of 100 spores were then placed in the near vicinity of actively growing transformed carrot (Daucus carota L.) roots (approximately 70 mm long) on Petri plates (90 mm in diameter) containing the MSR medium, following the method described in Cranenbrouck et al (2005). The Petri plates were incubated for 3 months in the dark in an inverted position at 27°C.…”

Section: Biological Materialsmentioning

confidence: 99%

Transcriptional regulation of defence genes and involvement of the WRKY transcription factor in arbuscular mycorrhizal potato root colonization

Gallou

Declerck

Cranenbrouck

2011

Funct Integr Genomics

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The establishment of arbuscular mycorrhizal associations causes major changes in plant roots and affects significantly the host in term of plant nutrition and resistance against biotic and abiotic stresses. As a consequence, major changes in root transcriptome, especially in plant genes related to biotic stresses, are expected. Potato microarray analysis, followed by real-time quantitative PCR, was performed to detect the wide transcriptome changes induced during the pre-, early and late stages of potato root colonization by Glomus sp. MUCL 41833. The microarray analysis revealed 526 up-regulated and 132 down-regulated genes during the pre-stage, 272 up-regulated and 109 down-regulated genes during the early stage and 734 up-regulated and 122 down-regulated genes during the late stage of root colonization. The most important class of regulated genes was associated to plant stress and in particular to the WRKY transcription factors genes during the pre-stage of root colonization. The expression profiling clearly demonstrated a wide transcriptional change during the pre-, early and late stages of root colonization. It further suggested that the WRKY transcription factor genes are involved in the mechanisms controlling the arbuscular mycorrhizal establishment by the regulation of plant defence genes.

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“…In monoxenic cultures of F. mosseae, sporulation was observed 15-20 d after association with transformed linum roots and continued up to 7 mo. The monoxenically cultured spores were isolated by solubilization of the MSR medium [32] and used for storage studies.…”

Section: Am Fungal Inoculamentioning

confidence: 99%

Maximizing the Shelf Life of Monoxenically Produced Carrier-Based Am Fungal Bio-Inocula for Maintaining Its Long-Term Viability

Rodrigues

Rodrigues²

2018

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Arbuscular mycorrhizal (AM) fungi are often applied as bio-inoculants due to their plant growth promoting benefits. The objectives of the present work were to maximize the shelf life of monoxenically produced carrier-based AM fungal bio-inocula of Rhizoglomus intraradices and Funneliformis mosseae. Shelf life of in vitro produced inoculum was studied by assessing the infectivity potential of in vitro produced inocula in an optimum carrier formulation (vermiculite: cow dung powder: wood powder: wood ash in the ratio of 20:8:2:1) during storage at three different temperatures viz., 4 °C, 25 °C and room temperature (RT). The re-germination potential of in vitro produced spores from carrier-based inocula to in vitro conditions was also examined. The in vitro produced inocula stored at 25 °C remained viable up to 6 mo in the organic carrier formulation. 100 % germination was recorded when the spores of both AM species were cultured back to in vitro conditions indicating high viability, and efficiency of the carrier formulation in maintaining vigour of in vitro produced propagules.

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Methodologies for in Vitro Cultivation of Arbuscular Mycorrhizal Fungi with Root Organs

Cited by 83 publications

References 41 publications

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

The induction of Ethylene response factor 3 (ERF3) in potato as a result of co-inoculation withPseudomonassp. R41805 andRhizophagus irregularisMUCL 41833 – a possible role in plant defense

Transcriptional regulation of defence genes and involvement of the WRKY transcription factor in arbuscular mycorrhizal potato root colonization

Maximizing the Shelf Life of Monoxenically Produced Carrier-Based Am Fungal Bio-Inocula for Maintaining Its Long-Term Viability

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