Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

Moreira, Mônica; Noschang, Juliana; Neiva, Ivana Froede; Carvalho, Yanê; Higuti, llma Hiroko; Vicente, Vânia Aparecida

doi:10.1590/s1516-89132010000400013

Cited by 17 publications

(12 citation statements)

References 19 publications

(14 reference statements)

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“…A total of sixty three B. cereus isolates were cultured in nutrient agar and then extraction of genomic DNA were made according to the protocol adapted from Moreira et al (2010). The concentration of the obtained DNA were determined using a spectrophotometer (Nanodrop 2000, Thermo Scientific, Wilmington-MA, USA) and dilutions were made, when necessary, to set the concentration at 100 μg/mL.…”

Section: Methodsmentioning

confidence: 99%

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil

Reis

Montanhini

Bittencourt

et al. 2013

Braz. J. Microbiol.

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Bacillus cereusis an ubiquitous, spore-forming bacteria that can survive pasteurization and the majority of the heating processes used in the dairy industry. Besides, it is a pathogen responsible for different types of food poisoning. One type of foodborne disease caused by B.cereusis the diarrheal syndrome, which is caused by the ingestion of vegetative cells producing toxins in the small intestine. One virulence factor for the diarrheal syndrome is the toxin hemolysin BL (HBL), a three-component protein formed by the L1, L2 and B components. In order to evaluate the presence of diarrheal strains isolated from milk and dairy products, 63 B. cereus isolates were obtained from 260 samples of UHT milk, pasteurized milk and powdered milk, sold in commercial establishments and from different brands. The isolates were subjected to the Polymerase Chain Reaction (PCR) for the detection of the encoding genes for the L1, L2 and B components and the toxin production capacity were evaluated with an immunoassay. A total of 23 [36.5%] isolates were identified carrying simultaneously the three tested genes, from which, 20 [86.9%] showed toxigenic capacity. 26 [41.3%] isolates did not carry any of genes tested and the other 14 [22.2%] were positive for one or two of them. The results showed a high toxigenic capacity among the B. cereus isolates able to produce the HBL, indicating a potential risk for consumers.

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Section: Methodsmentioning

confidence: 99%

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil

Reis

Montanhini

Bittencourt

et al. 2013

Braz. J. Microbiol.

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“…Overnight cultures of single pure colonies in nutrient broth were used for DNA extraction according to the protocol of Moreira et al (2010). DNA quantification was performed using Qubit (Invitrogen  ).…”

Section: Isolation and Identification Of Microorganismsmentioning

confidence: 99%

Screening for quality indicators and phenolic compounds of biotechnological interest in honey samples from six species of stingless bees (Hymenoptera: Apidae)

et al. 2017

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Honey from stingless bees of the genus Melipona is a well sought product. Nevertheless lack of legal frameworks for quality assessment complicates the evaluation of food safety and marketing of these products. Seeking to assess the quality of honey from the bees of this genus, physical and chemical analyses, identification of phenolic compounds, and microbiological evaluation from six species of stingless bees was performed. The honey samples showed high reducing sugars, low protein levels and a balanced microbiota. High total phenols and flavonoids and higher antioxidant activity were also recorded. Different phenolic compounds of great biotechnological potential were identified and of these apigenin, kaempferol and luteolin were identified for the first time in honey. To the best of our knowledge, this is one of the few works describing a detail characterization of melipona honey together with identification of the phenolic compounds of significant therapeutic value.Keywords: honey; chromatography; flavonoids; phenols; antioxidants; stingless bees; pollen types.Practical Application: This is one of the few works that describe the characterization of honey from seven species of stingless bees together detailing the identification of phenolic compounds of significant therapeutic value, which can serve as an important reference for future studies.

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“…Para bactérias a extração de DNA foi realizada de acordo com o protocolo de Moreira et al (2010). Os isolados foram cultivados em Caldo Nutriente e após o período de incubação, as culturas foram centrifugadas a 49000 x g por 2 min e o sedimento transferido para um microtubo de volume 1,5mL, contendo uma mistura de sílica em pó e celite 2:1, e tampão CTAB.…”

Section: Identificação Molecularunclassified

Uso De Dados Genômicos Como Indicadores De Identidade E Qualidade Na Gestão De Coleções Microbiológicas

Almeida¹,

Santos²,

Nascimento³

et al. 2019

Inventário De Recursos Genéticos

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Methodological variations in the isolation of genomic DNA from Streptococcus bacteria

Cited by 17 publications

References 19 publications

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil

Gene detection and toxin production evaluation of hemolysin BL of Bacillus cereus isolated from milk and dairy products marketed in Brazil

Screening for quality indicators and phenolic compounds of biotechnological interest in honey samples from six species of stingless bees (Hymenoptera: Apidae)

Uso De Dados Genômicos Como Indicadores De Identidade E Qualidade Na Gestão De Coleções Microbiológicas

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