Methodological Aspects of Measuring Human Skeletal Muscle Electrolyte Content and Ouabain Binding Capacity

Djurhuus, M. S.; Klitgaard, N. A.; Tveskov, Claus; Madsen, Klavs; Guldager, B; Jelnes, R; Petersen, P. H.; Beck‐Nielsen, Henning

doi:10.1006/abio.1998.2625

Cited by 11 publications

(8 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This might reduce sample size and improve analytical precision, which would be especially desirable in human studies where such invasive procedures should be limited to minimum. Consistent with this idea, freeze-drying was recently reported to reduce variation in measurement of electrolyte concentrations and the ouabain binding capacity in human skeletal muscle [10]. Freeze-drying of muscle biopsies also permits precise division of the biopsy sample into the desired aliquots of essentially homogenous material, thus allowing better matching of results between separate homogenizations.…”

Section: Introductionmentioning

confidence: 74%

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Korsheninnikova

Seppälä-Lindroos

Vehkavaara

et al. 2002

Diabetes Metabolism Res

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 74%

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Korsheninnikova

Seppälä-Lindroos

Vehkavaara

et al. 2002

Diabetes Metabolism Res

View full text Add to dashboard Cite

show abstract

“…Mg is reabsorbed in the loop and distal convoluted tubule but not in the collecting ducts that avidly reabsorb glucose. However, as both plasma Mg concentration and muscle Mg content are kept within narrow limits [18,24], it seems likely that some hormonal mechanisms control Mg homeostasis even though no single hormone with a sole effect on Mg homeostasis has been found so far. Blood glucose excursions might in¯uence such hormone(s).…”

Section: Resultsmentioning

confidence: 97%

Hyperglycaemia enhances renal magnesium excretion in Type 1 diabetic patients

Djurhuus

Skøtt

Vaag

et al. 2000

Scandinavian Journal of Clinical and Laboratory Investigation

View full text Add to dashboard Cite

Magnesium depletion is a common feature of diabetes mellitus, apparently related to glycaemic control. The aim of the study was to investigate the isolated effect of hyperglycaemia upon renal magnesium excretion. Urinary magnesium excretion rates were measured in 10 patients with Type 1 diabetes mellitus on two different days with different levels of blood glucose concentration but equal plasma insulin concentration. On a hyperglycaemic day, an i.v. infusion of 20% glucose was started at the end of a euglycaemic baseline period, increasing blood glucose concentration from 5.3 mmol/L to 12.3 mmol/L. There was no major glucosuria. On the hyperglycaemic day the renal magnesium excretion and clearance were raised by a factor of 2.4 compared to the euglycaemic day. Plasma magnesium concentration decreased 3% during hyperglycaemia. In conclusion, blood glucose excursions influence magnesium homeostasis independently of insulin levels in Type 1 diabetic patients. This effect is primarily due to an increased renal magnesium clearance during hyperglycaemia.

show abstract

“…In some studies, an attempt to correct for extracellular sodium in the measurement of the intracellular sodium We have previously shown that total muscle electrolyte content measured on small, human content has been made by calculating the chloride space [2][3][4][5][6]. In most of these studies, the biopsies should be performed after drying and dissection of the biopsy specimens [1]. However, intracellular chloride content is calculated on the assumption that there is a normal resting the coeYcient of variation in the determination of muscle sodium content is above 30% [1].…”

mentioning

confidence: 99%

“…In most of these studies, the biopsies should be performed after drying and dissection of the biopsy specimens [1]. However, intracellular chloride content is calculated on the assumption that there is a normal resting the coeYcient of variation in the determination of muscle sodium content is above 30% [1]. membrane potential in the muscles, but the resting membrane potential is subject to changes Some of this variation must be due to the with changes in electrolyte content.…”

mentioning

confidence: 99%

“…The biopsy intracellular and extracellular sodium in small, specimens were then dissected free of connective human muscle biopsy specimens using pretissue, fat, interstitial uid and blood. The disviously published data [1] to determine whether sected weight was determined, corrected for the measured intramuscular sodium content re ects uptake of water during weighing, and used in extra-or intracellular sodium. the calculation of the electrolyte content.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Measured muscle sodium content in biopsy specimens is a reflection of true intracellular content

Djurhuus

Klitgaard

Petersen

2002

Scandinavian Journal of Clinical and Laboratory Investigation

View full text Add to dashboard Cite

Determined on the basis of small skeletal muscle biopsies, muscle sodium content has a very high coefficient of variation. Furthermore, at least some of the measured sodium must originate from the extracellular space. In order to assess the applicability of the measurement of intracellular sodium on small muscle biopsy specimens, the measured sodium content was related to the content of dry solids in 25 muscle biopsy samples, and compared with the theoretical content of sodium with varying extra- and intracellular water content in biopsy samples. Four of the 25 measurements were clearly outliers. Disregarding these outliers, it was found that muscle sodium content varied with intracellular water content, whereas the theoretical effect of addition of extracellular water could not account for the observed values. The difference depended upon the specified conditions, but the slope of the theoretical regression line (-25.92 mmol x (kg dry weight)(-1) x %(-1), which was closest to the observed slope, -8.55 mmol x (kg dry weight)(-1) x %(-1), differed substantially (p < 0.0001). No association was found between the primarily intracellular ions muscle potassium and muscle magnesium on the one hand and either muscle sodium or muscle water content on the other. The measured sodium content in muscle biopsy specimens, which are freeze-dried and dissected, seems to reflect the true intracellular sodium content to some extent. The total content of sodium seems to be closely related to the content of water within the skeletal muscle cells.

show abstract

Methodological Aspects of Measuring Human Skeletal Muscle Electrolyte Content and Ouabain Binding Capacity

Cited by 11 publications

References 13 publications

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Elevated fasting insulin concentrations associate with impaired insulin signaling in skeletal muscle of healthy subjects independent of obesity

Hyperglycaemia enhances renal magnesium excretion in Type 1 diabetic patients

Measured muscle sodium content in biopsy specimens is a reflection of true intracellular content

Contact Info

Product

Resources

About