Methodological Approaches to Detection of Tamm-Horsfall Protein

Recently, Ciphergen Biosystems (Fremont, CA, USA) has developed a technique called surface-enhanced laser desorption/ionization (SELDI). This technology is based on ProteinChips(R) with chemically or biochemically modified surfaces for the selective retention and enrichment of protein subsets from a complex protein mixture. The proteins or peptides of interest can then be identified by using mass spectrometry (SELDI-MS). This highly sensitive and high-throughput technique can detect minute differences in proteins and peptide profiles between biological samples. The versatility of this technology enables a wide variety of applications in basic research, clinical, proteomic, and drug discovery such as identification of novel biomarkers associated with certain diseases or treatments. Many disease biomarkers or biomarker patterns have been identified using SELDI-MS in various laboratories. In this chapter, we provide a general guide to the profiling of proteins or peptides as well as biomarker discovery using the most common body fluids such as serum/plasma, nipple aspiration fluid, and urine.

show abstract

Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body Fluids

Gagnon

Shi

2008

Methods in Molecular Biology

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Methodological Approaches to Detection of Tamm-Horsfall Protein

Abstract: A new test system for measuring Tamm-Horsfall protein was developed. The system consists of antigenic diagnostic agent and immune serum. The specificity and sensitivity of the reagents were determined. The system was tested on 52 urine samples from subjects without renal diseases.

Cited by 1 publication

References 9 publications

Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body Fluids

Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry for Protein and Peptide Profiling of Body Fluids

Contact Info

Product

Resources

About