Methodological advances for selenium speciation analysis in yeast

Encinar, Jorge Ruiz; Śliwka-Kaszyńska, Magdalena; Polatajko, Aleksandra; Vacchina, Véronique; Szpunar, Joanna

doi:10.1016/s0003-2670(03)00754-2

Cited by 78 publications

(60 citation statements)

References 23 publications

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“…Therefore, estimation of adequate levels of Se in the diet requires information not only of the total amount of Se but also of the bio-accessible species in the sample [6,23,24]. Seenriched yeast has been extensively studied and fractionated [12,25] with SeMet reported as the major selenoamino acid in water-soluble yeast extracts, replacing Met by simple competition. Se yeast products consist of dried and inactivated preparations of S. cerevisiae; the final product is a light to dark brown, free-flowing powder readily dispersible in water and organic solvents [26][27][28].…”

Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

“…Se yeast products consist of dried and inactivated preparations of S. cerevisiae; the final product is a light to dark brown, free-flowing powder readily dispersible in water and organic solvents [26][27][28]. Aqueous extraction allows the recovery of 15-25 % of Se [12] present predominately as protein-bound SeMet [29,30]; therefore, an enzymatically assisted extraction method is typically used to determine total SeMet content [11]. Essentially, larger SeMet-containing proteins and peptides are broken down into free amino acids and short-chain peptides liberating the selenoamino acid for detection [19].…”

Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

“…It has been proposed that some SeMet in yeast may not be incorporated chemically into Se-containing proteins but physically associated with cell wall constituents [3,23]. As commercial Se yeast preparations consist of dried and inactivated fragments of S. cerevisiae, it is reasonable to assume that some leaching may occur post-production and prior to drying [12].…”

Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

“…Proteins containing Se in the form of SeMet residues are not formally classified as selenoproteins but rather called selenium-containing proteins [12]. Therefore, in the case of organic Se products such as Se yeast, biological efficacy is more dependent on the digestibility and accessibility of Secontaining proteins and peptides present in commercial preparations.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, in the case of organic Se products such as Se yeast, biological efficacy is more dependent on the digestibility and accessibility of Secontaining proteins and peptides present in commercial preparations. An assessment of the composition of Se yeast products in the marketplace indicated that significant differences exist between the products [13]; however, speciation studies traditionally focused on the determination of low molecular mass selenocompounds [12].…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Comparison of Commercial Selenium Yeast Preparations

Fagan

Owens

Ward

et al. 2015

Biol Trace Elem Res

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The trace mineral selenium (Se) is an essential element for human and animal nutrition. The addition of Se to the diet through dietary supplements or fortified food/feed is increasingly common owing to the often sub-optimal content of standard diets of many countries. Se supplements commercially available include the inorganic mineral salts such as sodium selenite or selenate, and organic forms such as Se-enriched yeast. Today, Se yeast is produced by several manufacturers and has become the most widely used source of Se for human supplementation and is also widely employed in animal nutrition where approval in all species has been granted by regulatory bodies such as the European Food Safety Authority (EFSA). Characterisation and comparison of Se-enriched yeast products has traditionally been made by quantifying total selenomethionine (SeMet) content. A disadvantage of this approach, however, is that it does not consider the effects of Se deposition on subsequent digestive availability. In this study, an assessment was made of the water-soluble extracts of commercially available Se-enriched yeast samples for free, peptide-bound and total water-soluble SeMet. Using LC-MS/MS, a total of 62 Se-containing proteins were identified across four Se yeast products, displaying quantitative/ qualitative changes in abundance relative to the certified reference material, SELM-1 (P value <0.05; fold change ≥2). Overall, the study indicates that significant differences exist between Se yeast products in terms of SeMet content, Secontaining protein abundance and associated metabolic pathways.

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Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

Section: Determination Of Total Free and Bound Selenomethioninementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biochemical Comparison of Commercial Selenium Yeast Preparations

Fagan

Owens

Ward

et al. 2015

Biol Trace Elem Res

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Recent Advances in Analytical Chemistry and Mass Spectrometry for Organic Selenium and Tellurium Compounds

Far

Pauw

Eppe

2011

Patai's Chemistry of Functional Groups

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The objective of this chapter is to present the recent developments in mass spectrometry for selenium (Se) and tellurium (Te) analysis. The state‐of‐the‐art of mass spectrometry‐based techniques available is critically evaluated with particular emphasis on ion sources and mass analyzers. The complexity of identification and characterization of Se, Te species including their speciation in the environment or in biological materials requires methodologies not only limited to mass spectrometry. Particular attention is given to sample preparation and hyphenated techniques with mass spectrometry for a comprehensive characterization of these trace elements. All these aspects are illustrated with up‐to‐date applications from the literature. Finally, future trends in mass spectrometry give the readers new insights into Se and Te species detection.

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Enhanced Antioxidant Formula Based on a Selenium‐Supplemented Carotenoid‐Producing Yeast Biomass

Breierová

Gregor

Márová

et al. 2008

Chemistry & Biodiversity

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Carotenoid-producing yeast species such as Rhodotorula glutinis and Sporobolomyces roseus efficiently accumulated selenium from the growth medium. It was observed that incorporation of selenium into yeast cells during the growth inhibited production of beta-carotenoid and other carotenoid precursors (torularhodin and torulene). The yeasts with high content of the carotenoid pigments and selenium may be used for the preparation of a new type of antioxidant formula that could be directly applied for various human and animal diets. We have demonstrated that such a formula can only be produced by separate processes of the cultivation of red yeasts and a subsequent sorption of selenium into the cells.

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Methodological advances for selenium speciation analysis in yeast

Cited by 78 publications

References 23 publications

Biochemical Comparison of Commercial Selenium Yeast Preparations

Biochemical Comparison of Commercial Selenium Yeast Preparations

Recent Advances in Analytical Chemistry and Mass Spectrometry for Organic Selenium and Tellurium Compounds

Enhanced Antioxidant Formula Based on a Selenium‐Supplemented Carotenoid‐Producing Yeast Biomass

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