Méthodes d’identification et de quantification des moisissures de l’habitat : méthodes classiques, méthodes moléculaires

Fréalle, Émilie; Bex, Valérie; Reboux, Gabriel; Roussel, Sandrine; Bretagne, Stéphane

doi:10.1016/j.rmr.2017.01.009

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…The quality of indoor air has become a subject of great interest as people are spending most of their time indoors ( 1 ). Biological pollutants such as fungi can, however, affect this air quality and can cause adverse health effects as fungal spores, mycotoxins, fungal allergens, and fungal volatile organic compounds can induce allergic, toxic or infectious effects ( 2 , 3 ). The genera Aspergillus, Cladosporium, Penicillium, Alternaria , and yeasts are the most common fungi found indoors ( 4 ).…”

Section: Introductionmentioning

confidence: 99%

“…Identification of molds found in the indoor environment is generally performed using microscopy. This standard method has, however, some limitations as it is time consuming and it needs mycologists with high expertise while identification is often limited to the genus level ( 2 , 13 ). Molecular methods can offer relief ( 2 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

“…This standard method has, however, some limitations as it is time consuming and it needs mycologists with high expertise while identification is often limited to the genus level ( 2 , 13 ). Molecular methods can offer relief ( 2 , 14 ). However, DNA based techniques such as polymerase chain reactions are limited in the panel of species they can identify and multi-locus gene sequencing (the “gold standard” for the identification of microfungi) is very expensive, time consuming and prone to environmental contamination.…”

Section: Introductionmentioning

confidence: 99%

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Implementation of MALDI-TOF Mass Spectrometry to Identify Fungi From the Indoor Environment as an Added Value to the Classical Morphology-Based Identification Tool

Motteu¹,

Goemaere²,

Bladt³

et al. 2022

Front. Allergy

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IntroductionDuring the last decades, molds in the indoor environment have raised concern regarding their potential adverse health effects. The genera Aspergillus, Cladosporium, Penicillium, Alternaria, and yeasts, the most common fungi found indoors, include species with high allergenic and toxigenic potentials. Identification of these molds is generally performed by microscopy. This method has, however, some limitations as it requires mycologists with high expertise while identification is often limited to the genus level. Therefore, it is necessary to seek for fast and accurate tools, such as Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDITOF MS), enabling an identification to the species level and guiding general practitioners in their search for the underlying cause of a health problem.MethodsIn this study, 149 fungal air and dust isolates from 43 dwellings in Brussels were taken in collaboration with Brussels Environment RCIB/CRIPI and identified by both microscopy and MALDI-TOF MS in Sciensano's Indoor Mycology laboratory. Spectra obtained via MALDI-TOF MS were compared with data available in an in-house created reference database containing over 1,700 strains from the BCCM/IHEM fungal collection.ResultsA total of 149 isolates including 18 yeasts and 131 filamentous fungi were analyzed. Microscopic analysis indicated 18 yeast species and allowed identification of 79 isolates (53%) to the genus level. Only 36 isolates (24%) could be identified to the species complex level. Fifteen molds (10%) could not be identified, and one was indicated as sterile mycelia. No isolate was identified to species level. MALDI-TOF MS analysis identified 137 (92%) of the 149 isolates with a logscore > 1.7. Of these 137 isolates, 129 (87%) were identified to the species level (logscore > 2.0). For only 8 isolates (5%), identification was limited to the genus/section level (1.7 < logscore <2.0), and 12 isolates (8%) could not be identified.ConclusionA comparison of results obtained with both methods indicates an increased precision in identifications with MALDI-TOF MS analysis for 92% of the isolates, emphasizing its highly added value to the standard microscopic analysis in routine practice. In addition, MALDI-TOF MS also enables to assess the accuracy of microscopic identifications.

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