Methoden der Biophysikalischen Chemie

Abstract

Die Studienbücher der Reihe Chemie sollen in Form einzelner Bausteine grundlegende und weiterführende Themen aus allen Gebieten der Chemie umfassen. Sie streben nicht die Breite eines Lehrbuchs oder einer umfangreichen Monographie an, sondern sollen den Studenten der Chemieaber auch den bereits im Berufsleben stehenden Chemiker -kompetent in aktuelle und sich in rascher Entwicklung befindende Gebiete der Chemie einführen. Die Bücher sind zum Gebrauch neben der Vorlesung, aber auch -da sie häufig auf Vorlesungsmanuskripten beruhen -anstelle von Vorlesungen geeignet. Es wird angestrebt, im Laufe der Zeit alle Bereiche der Chemie in derartigen Lehrbüchern vorzustellen. Die Reihe richtet sich auch an Studenten anderer Naturwissenschaften, die an einer exemplarischen Darstellung der Chemie interessiert sind.

Temperature‐Induced Unfolding of Ribonuclease A Embedded in Spherical Polyelectrolyte Brushes

“…Wave numbers of individual structure elements within the amide-I region according to ref. [43] . bands at 1678 and 1697 cm À1 appear with a minimum inbetween at 1691 cm À1 .…”

“…[41][42][43] The transition temperatures and Van't Hoff enthalpies of the free RNase A in solution were derived from the absorption at 1643 cm À1 and the position of the maximum of the amide-I band (Figure 7 and 8). In case of the adsorbed protein only the decrease in the adsorption at 1643 cm À1 was taken into account as a second maximum of the amide-I band occurs in the spectra at 45 8C (see Figure 5) which would pretend a wrong transition temperature.…”

“…However, there is a clear indication that the Van't Hoff enthalpy is significantly lowered by the adsorption to SPB. As the Van't Hoff enthalpy is a product of the calorimetric enthalpy and the cooperativity of the process [43] either a destabilization of the protein structure or an increase in the cooperativity of the process can be concluded. Future studies by calorimetry should allow further interpretation of the data.…”

Thermodynamic and structural behaviour of equimolar POPC/CnE4 (n=8, 12, 16) mixtures by sorption gravimetry, 2H NMR spectroscopy and X-ray diffraction

“…Therefore, the information on the lyotropic phase state, usually obtained by diffraction or spectroscopic methods (Winter et al, 1998) is substantial for the interpretation of results derived from non-structural methods. In our case, as a tool to assign mesophase structures to the respective samples, 31 P NMR (Cullis and Dekruijff, 1979) and X-ray diffraction were used.…”

Chiral Quadridentate Ligands Derived from Amino Acids and Some Zinc Complexes Thereof

“…[48] It is interesting to note that the differential extinction coefficients observed in this work are comparable with values obtained for biomolecules such as proteins or DNA. [51] …”