Methode zur Impr�gnation des in Paraffin eingebetteten Nervengewebes

Kelemen, J

doi:10.1007/bf00690679

Cited by 10 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell numbers for adult animals were obtained from 9-m-thick paraffin sections. All preparations were stained for Nissl substance (Kelemen, 1965). Only neurons containing a visible nucleolus were counted.…”

Section: Counting Of Neuron Numbersmentioning

confidence: 99%

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Koza

Wilhelm

Hiripi

et al. 2006

J of Comparative Neurology

View full text Add to dashboard Cite

Organization of the serotonergic system and changes of the serotonin (5-HT) content were studied during the embryogenesis of the earthworm Eisenia fetida, using immunocytochemistry and HPLC. A gradual emergence of 5-HT immunoreactive (IR) cells and their axon projections in the several ganglia of the central (CNS) and peripheral nervous system are described in the context of a staged time-scale of development. The first 5-HT-IR neurons appear in the subesophageal ganglion at an early embryonic stage (E2), followed by neurons in some rostrally located ventral ganglia. In the cerebral ganglion, 5-HT-IR cells can be detected only from stage E5. The number of labeled cells in each ganglion of the embryo increases until hatching, when it is still considerably lower than that observed in adults. This shows that the development of the 5-HTergic system is far from complete by the end of embryogenesis. Organization of 5-HT-IR innervation of the body wall starts by stages E3 to E4. In the stomatogastric nervous system the first 5-HT-IR fibers can be detected by stage E5. By stage E9 5-HT immunopositive neurons can be observed in both the stomatogastric ganglia and the enteric plexus. Both 5-HT levels and the numbers of the labeled cells show a significant increase before hatching, which indicate a functional maturation of the 5-HTergic system. Based on the early appearance of 5-HT, we suppose that it may play a regulatory role in both the gangliogenesis and the maturation of peripheral functions necessary during postembryonic life.

show abstract

Section: Counting Of Neuron Numbersmentioning

confidence: 99%

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Koza

Wilhelm

Hiripi

et al. 2006

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…Serial sections of both intact and regenerated brain were stained with toluidine blue solution [20]. Only nerve cells containing a well-visible nucleus were counted.…”

Section: Cell Countingmentioning

confidence: 99%

Reorganization of the GABAergic system following brain extirpation in the earthworm (Eisenia fetida,Annelida, Oligochaeta)

Csoknya¹,

Barna²,

Elekes³

2002

Acta Biologica Hungarica

View full text Add to dashboard Cite

The reorganization of the GABAergic system was studied by means of immunohistochemistry after the symmetrical and asymmetrical (unilateral) extirpation of the brain of the annelid Eisenia fetida. GABA-immunoreactive neurons were first observed in the wound tissue on the 3rd postoperative day. Thereafter the number of labelled cells gradually increased, and by postoperative days 76-80 all GABA-immunoreactive cells (approx. 140 neurons) could be found in their final positions in the symmetrically regenerated brain. After asymmetrical brain extirpation, nearly all cells (70-75) could be detected in the regenerating hemisphere by postoperative days 50-56. In the early stages of the asymmetrical regeneration of the brain, more GABAergic cells were concentrated dorsally and laterally in the preganglion than during the symmetrical type of regeneration. In both types of regeneration, the immunoreactive neurons in the regenerated brain originated in part from undifferentiated neuroblasts situated in different parts of the body, and in part from dividing neurons localized mainly in the pharyngeal nerve plexus. Both exogenous GABA and picrotoxin, applied during the early stages (days 10- 12) of brain regeneration, inhibited the development of the wound tissue and the migration of the neuroblasts and the enteric neurons. At the same time, exogenous GABA application accelerated the proliferation of the pharyngeal neurons. No effect on the process of regeneration could be demonstrated when exogenous GABA and picrotoxin were given together.

show abstract

“…Light microscopy of the human lamina cribrosa region, in eyes with a history of glaucoma and raised IO P (a, b from case 10 in table 1; c-f from case 11). a The material was embedded in paraffin and impregnated with silver, following Kelemen [12], Nu merous apparently degenerating axon profiles can be seen, and one is indicated by an arrow, which also points towards the retina. Scale bar = 100 pm.…”

Section: Rabbitmentioning

confidence: 99%

Evidence of Constriction of Optic Nerve Axons at the Lamina cribrosa in the Normotensive Eye in Humans and Other Mammals

et al. 1995

View full text Add to dashboard Cite

The ultrastructure of optic nerve axons was examined in several mammals (human, cat, rat, sheep, ox, pig, guinea pig, rabbit). Human material was obtained from normotensive, glaucoma-free eyes and from eyes with a history of glaucoma and raised intra-ocular pressure (IOP). We describe accumulations of organelles (principally mitochondria) in optic nerve axons where they traverse the lamina cribrosa. Accumulations were most prominent in unmyelinated lengths of axons close to lamellae of the lamina cribrosa. Comparable accumulations were not apparent in axons in the retina or optic nerve, suggesting that axoplasmic flow is constricted at the lamina cribrosa. Accumulations were observed both centrally and peripherally to the lamellae, suggesting that flow is constricted in both ortho- and antero-grade directions. Accumulations of organelles were more marked in unmyelinated axons than in adjacent, myelinated axons. In the rabbit, in which most axons are myelinated as they traverse the optic nerve head, organelle accumulations were observed only in a sparse population of unmyelinated axons. In human eyes with a history of raised IOP and glaucoma, the accumulations were abnormally large and frequent and in many axons showed dense-body and fibrillar changes not seen in normotensive eyes. It is suggested that chronic, partial constriction of axoplasmic flow is present at the lamina cribrosa of normotensive eyes in a wide range of mammals, including humans, that the constriction results from the pressure gradient across the lamina cribrosa and that the constriction may be a factor in the many cases of primary glaucoma in which IOP is not raised.

show abstract

Methode zur Impr�gnation des in Paraffin eingebetteten Nervengewebes

Cited by 10 publications

References 7 publications

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Embryogenesis of the serotonergic system in the earthworm Eisenia fetida (Annelida, Oligochaeta): Immunohistochemical and biochemical studies

Reorganization of the GABAergic system following brain extirpation in the earthworm (Eisenia fetida,Annelida, Oligochaeta)

Evidence of Constriction of Optic Nerve Axons at the Lamina cribrosa in the Normotensive Eye in Humans and Other Mammals

Contact Info

Product

Resources

About