Methode zur Bestimmung des intracellulären Säurebasenhaushaltes (pH, pCO2, Standardbicarbonat, Basenüberschuß) in Erythrocyten

Gleichmann, U.; Stuckrad, H. v.; Zindler, M.

doi:10.1007/bf00363299

Cited by 21 publications

(1 citation statement)

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“…1) the so-called 'liquid junction potential" (13), 2) ghicose breakdown by glycolysis (14) or via the hexose monophosphate shunt (15), 3) a CO 2 shift during the centrifugation (if the temperature of 37 °C is not maintained) (13).…”

Section: Discussionmentioning

confidence: 99%

Methods for pH Determination in Human Erythrocytes

Büttner

Büttner²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:The methods described in the literature for pH determination in human erythrocytes, i.e. the direct potentiometric measurement of haemolysed erythrocytes and the 5,5-dimethyl-2,4-oxazolidinedione (DMO) method, were examined and compared. In spite of careful optimization of the experimental technique, a statistically significant difference of a few hundredths of a pH unit remained between the results of the two methods. The calculation of the cellular water space of the erythrocytes is suggested äs a possible reason for this difference; in the DMO method this leads to uncertainty in the determination of the DMO concentration and therefore in the derived intracellular pH values. For clinical use, the direct pH measurement of haemolysed erythrocytes, using the technique described here, is recommended.

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Section: Discussionmentioning

confidence: 99%