Method validation of 16 types of structural amino acids using an automated amino acid analyzer

Shim, You-Shin; Yoon, Won Jin; Ha, Jaeho; Seo, Dongwon; Lee, Kwang Won; Lee, Woo Young; Kwon, Kwang Il; Kang, Tae Seok; Lee, Jin Ha; Kim, Hyun Jeong; Kwak, Hae Jung; Lee, Sam Pin; Kim, So Jung; Yun, Won Kap; Lee, Junsoo; Hwang, Jin Bong

doi:10.1007/s10068-013-0252-0

Cited by 28 publications

(13 citation statements)

References 26 publications

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“…LOD values ranged from 0.06 g kg -1 to 0.72 g kg -1 (Table 1). Similar LOD values were obtained by Jajić et al [5] during an analysis of 14 AAs in feeds with HPLC method (0.02 g kg -1 to 1.30 g kg -1 ) and by Shim et al [11] (0.07 g kg -1 to 0.59 g kg -1 ) analysing 16 AAs in infant formula by UPLC method using AQC for pre-column derivatization. The precision of AAs measurement (repeatability and within-laboratory reproducibility) in investigated rye, pork haemoglobin and feed mixture samples was evaluated using relative standard deviation of replicated measurements (n = 10).…”

Section: Resultssupporting

confidence: 82%

“…3. Good chromatographic separation was obtained for all AAs, including sulphur AAs, which is an advantage of proposed procedure, as not all sulphur AAs and secondary AAs were determined with the use of pre-column derivatization [5,11]. The validation of analytical procedure for determination of AAs in rye, pork haemoglobin and feed mixture samples converted determination of all parameters required in the feed control area.…”

Section: Resultsmentioning

confidence: 94%

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Method validation for determination of amino acids in feed by UPLC

et al. 2017

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The paper presents results of validation of an analytical procedure based on ultra-performance liquid chromatography technique (UPLC) for determination of 17 amino acids in different feeds. The following performance characteristics were determined for the investigated feeds: relative standard deviations of repeatability and intermediate precisions ranged from 0.4 % to 4.6 %, and from 0.8 % to 7.9 %, respectively; recovery rates ranged from 87 % to 104 %, and limit of detection was from 0.06 g kg -1 (methionine) to 0.72 g kg -1 (glutamic acid). Two approaches were used to estimate measurement uncertainty giving values in a range of 5.1 % to 5.5 %. These performance characteristics are in agreement with the values reported in Commission Regulation (EU) No 152/2009 for the ion exchange chromatography with spectrophotometric detection.

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Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 94%

Method validation for determination of amino acids in feed by UPLC

et al. 2017

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“…The automated amino acid analyzer was validated using standards. The limits of detection and limits of quantification were less than 0.059 and 0.198 mg g -1 , respectively (Shim et al 2013).…”

Section: Methodsmentioning

confidence: 96%

Enzyme activity, amino acid profiles and hydroxymethylfurfural content in Ethiopian monofloral honey

Belay

Haki²,

Birringer

et al. 2017

J Food Sci Technol

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The enzymes activity, hydroxymethylfurfural (HMF) and amino acids in honeys are relatively low. However, they play very significant role for honey quality. In this study, enzymes, amino acids and HMF contents of Ethiopian monofloral honeys were investigated. Diastase, invertase and HMF were analyzed based on the Harmonized International Honey Commission method and amino acids using amino acids analyzer (HPLC). Diastase activity ranged from 3.91 ± 0.730 () to 13.6 ± 2.30 [ (L: Lalibella)]; invertase 36.5 ± 1.93 () to 4.85 ± 2.36 (); and HMF 0 ± 0 ( and ) to 3.37 ± 1.73 (). Significant variations were observed among honeys in diastase content, despite being from the same botanical origin. Significant variations were also observed among honeys in invertase and diastase contents. Bees' geographical race and location affected enzymes activities. Lower level of enzymes could be an intrinsic characteristic of Ethiopian honey. Thus, enzymes activity alone cannot be a worthwhile indicator of quality for Ethiopian honey; besides diastase and invertase activity, the quality control of Ethiopian honeys should be supported by HMF parameters.

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“…The sugar content of STPH was determined by the phenol/sulfonic acid method (Masuko et al ., ). The amino acid composition was analysed with the use of an amino acid analyser (L‐8900, Hitachi, Japan) according to the method previously described by Shim et al (). The total lipids were estimated according to the method of Folch et al ().…”

Section: Methodsmentioning

confidence: 99%

The soluble fraction of soy protein peptic hydrolysate reduces cholesterol micellar solubility and uptake

Jia

Tian

Zhang

et al. 2019

Int J of Food Sci Tech

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Summary Previous studies have demonstrated that the undigested or insoluble high molecular fraction of soy protein exerts a lowering effect on serum cholesterol by inhibiting the absorption of cholesterol in the intestinal tract. The aim of this study was to investigate the interaction between the soluble fraction of soy protein peptic hydrolysate and dietary mixed micelles (DMMs) for cholesterol micellar solubility and uptake. The collected soluble tofu peptic hydrolysate (STPH) components, generated from hard and lactone tofu were evaluated by two in vitro models and a Caco‐2 cell model after tofu soy protein was digested, thus simulating the gastrointestinal tract. The results showed that the administration of STPH effectively decreased the cholesterol solubility and uptake by 37.6% and 18.99%, respectively, as detected by the in vitro models and the Caco‐2 cell model. Current findings demonstrated the potential hypocholesterolaemic benefit of the STPH.

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Method validation of 16 types of structural amino acids using an automated amino acid analyzer

Cited by 28 publications

References 26 publications

Method validation for determination of amino acids in feed by UPLC

Method validation for determination of amino acids in feed by UPLC

Enzyme activity, amino acid profiles and hydroxymethylfurfural content in Ethiopian monofloral honey

The soluble fraction of soy protein peptic hydrolysate reduces cholesterol micellar solubility and uptake

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