Method Validation for the Determination of Eleutherosides and β-Glucan in Acanthopanax koreanum

Abstract

The aim of this study was to investigate the method validation for the determination of eleutherosides (B and E) and β-glucan in Acanthopanax (A.) koreanum. This medicinal plant reportedly mainly included eleutherosides which exhibit the pharmacological effects, and β-glucan substantially enhances the function of the immune system by activating macrophages. The specificity, linearity, precision, accuracy, limit of detection (LOD, S/N=3), and limit of quantification (LOQ, S/N=10) were measured by HPLC and enzymatic methods. Our results showed that the coefficient of calibration correlation (R 2) for eleutheroside B and E were 0.9997 and 0.9999, respectively. The limits of detection (LOD) for eleutheroside B and E were 0.050 μg/mL and 0.025 μg/mL, respectively. The recovery rate of eleutheroside B and E were revealed in the high range of 100.66～110.04% and 94.26～111.62%, respectively. The inter-day precision of eleutheroside B and E in the root and stem in A. koreanum were 1.4～5.0% and 1.1～2.5%, respectively. The intra-day precision of eleutheroside B and E in the root and stem in A. koreanum were 2.8～2.9% and 0.4～1.1%, respectively. Furthermore, the inter-day and intra-day precision of β-glucan in the stem, leaf, and fruit of A. koreanum were 1.32～5.67% and 8.01～11.76%, respectively. In conclusion, the methods were validated for the detection of eleutherosides and β-glucan in A. koreanum.

Eleutherosides Extraction from Acanthopanax sessiliflorus Seeman and Eleutherococcus senticosus Maxim Using an Enzymatic Process

“…오가피(Acanthopanax(A. ))는 인삼과 같은 두릅나무과에 속하는 낙엽활엽 관목으로 러시아, 중국, 일본, 한국 등에 분포하고 깊은 산지의 계곡에 서식하는 식물이다 (1)(2)(3)(4). 오 가피 품종으로 가시오가피(Eleutherococcus senticosus), 탐라오가피(A. koreanum), 지리오가피(A. chiisanensis), 오가피(A. sessiliflorus) 등이 있고 국내에 서식하는 오가피 는 14종이 알려졌다 (2,5).…”