Method validation for methanol quantification present in working places

Muna, E D M; Bizarri, C H B; Maciel, Jeanne; Rocha, Geovany; Araújo, I O de

doi:10.1088/1742-6596/575/1/012031

Cited by 2 publications

(2 citation statements)

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“…A technique that is available today and worth investigating in the bioproduction field is headspace analysis (HSA). Muna et al 16 elaborate in their paper on a highly sensitive, rapid, and reliable procedure for the quantification of methanol present in working environments through HSA. In this method, the quantification is performed by coupling the headspace injection with the GC flame ionization detection (GC–FID) method using a capillary column.…”

Section: Introductionmentioning

confidence: 99%

“…Several techniques have been recommended in the literature to quantify methanol in complex sample matrices. Some of these are (i) derivatization of sample matrices such as water–ethanol, 18 aqueous, 19 and alcohol; 20 (ii) direct injection of samples such as plasma, 21 whole blood, 22 alcohol, food products, 23 and wine; 24 (iii) HSA of wine, 25 washing filtrate, 26 and air; 16 (iv) solid-phase extraction of air; 27 and (v) dilution of cell cultures. 28 One of the most important and widely used techniques for the separation of a volatile component from biological samples is extraction.…”

Section: Introductionmentioning

confidence: 99%

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Processing Method for the Quantification of Methanol and Ethanol from Bioreactor Samples Using Gas Chromatography–Flame Ionization Detection

2022

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Methanol, a simple polar solvent, has been widely identified as an attractive carbon source to produce chemicals and fuels in bioprocesses. Specifically, to achieve recombinant protein production from methylotrophic yeasts, such as Pichia pastoris , this organic solvent can be used as a sole carbon source for growth and maintenance as well as an inducer for protein expression. However, if methanol feeding is not controlled well in such a fermentation process, accumulation of the solvent in the growth media will have a detrimental effect on the cells. Hence, monitoring the levels of methanol in these fermentation processes is a crucial step to ensure a healthy culture and maximum protein production. There are various techniques elaborated in the literature for monitoring methanol in cell cultures, but often, they appear to be expensive methods that are less affordable for many laboratories. This is because, in addition to the sophisticated equipment that is required for the analysis, the complexity of the samples retrieved from the bioprocesses necessitates laborious processing steps often involving expensive tools. In this study, a fast, simple, and sensitive method is developed to process biological samples by using the salting-out-assisted liquid–liquid extraction technique to quantify the concentration of methanol and ethanol using gas chromatography. On comparing the combinations of widely available salts and solvents, it was noticed that salting out using potassium carbonate followed by the liquid–liquid extraction of the analyte using ethyl acetate showed the best recovery. Followed by this, a validation test for the developed method was performed, which resulted in good peak resolution, linearity, and limit of detection for the quantitation of methanol and ethanol. By further assessing the tested combination, it was confirmed that its application could be extended to other matrices. Such an approach facilitates the possibility to monitor and control the methanol levels in fermentation and aids in bioprocess optimization.

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Section: Introductionmentioning

confidence: 99%