Method validation for analysis of phorbol esters from Jatropha curcas

Faria-Machado, A. F.; Licurgo, Flávia M.S.; Pires, Josi M.F.; Campos, Rodrigo da Silveira; Wilhelm, Allan Eduardo; Souza, Maria de Lourdes Mendes de; Antoniassi, Rosemar

doi:10.1016/j.indcrop.2019.111627

Cited by 8 publications

(6 citation statements)

References 13 publications

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“…There are several papers that show that phorbol esters have been detected in deoiled Jatropha cake. Faria-Machado et al 2 confirmed in their recent paper that phorbol esters were present in the jatropha seed cake that remained after oil extraction. These phorbol esters could be extracted using methanol as solvent.…”

mentioning

confidence: 85%

Critique on conclusions regarding toxic compounds in Jatropha curcas kernel cake

Francis¹,

Makkar

Carle

et al. 2021

Commun Biol

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mentioning

confidence: 85%

Critique on conclusions regarding toxic compounds in Jatropha curcas kernel cake

Francis¹,

Makkar

Carle

et al. 2021

Commun Biol

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“…An edible variety of Jatropha curcas is also available and known since centuries by local populations in Mexico (see Section 3.7). From a phenotypical and compositional point of view, this edible cultivar is similar to the non‐edible varieties (Makkar and Becker, 1997; Makkar et al, 2011; Valdes‐Rodríguez et al, 2013) but contains PEs at concentrations below limits of detection (LOD) by different analytical methods (LOD as low as 0.026 μg PEs/g kernel by HPLC‐UV) (Makkar et al, 1997; Martínez‐Herrera et al., 2010; Devappa et al, 2013; Baldini et al, 2014b; Faria‐Machado et al, 2019).…”

Section: Assessmentmentioning

confidence: 99%

Safety of hydrothermally treated kernels from edible Jatropha curcas L. (Chuta) as a novel food pursuant to Regulation (EU) 2015/2283

Turck¹,

Bohn²,

Castenmiller³

et al. 2022

EFS2

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Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on hydrothermally treated kernels from edible Jatropha curcas (Chuta) as a novel food (NF) pursuant to Regulation (EU) 2015/2283. Although Jatropha curcas is generally considered a toxic plant due to the presence of phorbol esters (PEs), edible varieties exist in Central America. The applicant has developed a breeding programme for an edible cultivar and proposes the kernels from this cultivar as an NF as whole kernels or fragments thereof to be used as a snack or as a food ingredient. Procedures are in place to avoid commingling with non‐edible kernels, with the last steps being the analytical control of PEs concentrations in all produced batches. The Panel considers that the production process of the NF is sufficiently described and that the information provided on the composition of the NF is sufficient for its characterisation. Components of the NF were tested for genotoxicity applying the standard in vitro test battery and no genotoxic concerns have been identified. In a conservative scenario for exposure to PEs from the NF, it was assumed that all kernels contain PEs at the level of detection of the analytical method. When comparing the estimated maximum exposure to PEs with a reference point from a subchronic study in pigs, a margin of exposure ≥ 900 is obtained, which is considered sufficiently large. The presence of anti‐nutritional factors does not pose safety concerns as they are within the ranges found in vegetables. The Panel concludes that the NF is safe under the proposed conditions of use.

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“…A study reported the reflux method as better than the ultrasonication method for extraction [36]. Another study found that among Soxhlet, direct extraction, and ultrasonication bath, Soxhlet was better in terms of efficiency and replicability of the process [44]. Near-infrared, microwave irradiation, ultrasound, and Soxhlet extraction were appraised for extraction of capsaicinoids and the study reported that Soxhlet extraction was the least effective [45].…”

Section: Optimization Of the Extraction Techniquesmentioning

confidence: 99%

Isolation, optimized extraction, and ultra‐high performance liquid chromatography with photodiode array method for quantitative analysis of chiratol in Swertia paniculata

Ahluwalia

Pandey

Mishra

et al. 2021

J of Separation Science

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The global natural product-based industry is growing fast with the introduction of new phytochemicals and herbal extract products from different geographical regions. Swertia paniculata is a well-known plant with medicinal properties; however, the quality control for its major phytochemical constituents from the Himalayan geographical region is nevertheless reported. Therefore, the first objective of this investigation was to characterize and optimize the extraction process while the second objective was to validate a quantitative analytical method for chiratol from S. paniculata herbal extract. The chiratol was characterized with spectral analysis. The optimum extraction condition for the highest yield of metabolite was realized in chloroform as a solvent system under ultrasonication. The ultra-high performance liquid chromatography coupled with photodiode array detection method for analytical quantification was validated for specificity, linearity, limits of detection, limits of quantification, precision, repeatability, recovery, and robustness using Eclipse Plus C 18 column (100 mm × 4.6 mm × 3.5 μm id). The gradient elution of water/acetonitrile as mobile phase was used at a flow rate of 0.5 ml/min. The recovery percentage was very satisfactory with values within specification. The robustness parameters showed no substantial influence of evaluated parameters by the Youden test. The developed method was ascertained to be appropriate for the proposed purpose.

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Method validation for analysis of phorbol esters from Jatropha curcas

Cited by 8 publications

References 13 publications

Critique on conclusions regarding toxic compounds in Jatropha curcas kernel cake

Critique on conclusions regarding toxic compounds in Jatropha curcas kernel cake

Safety of hydrothermally treated kernels from edible Jatropha curcas L. (Chuta) as a novel food pursuant to Regulation (EU) 2015/2283

Isolation, optimized extraction, and ultra‐high performance liquid chromatography with photodiode array method for quantitative analysis of chiratol in Swertia paniculata

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