Method to determine collagen density distributions in fibrous tissues

Abstract

We present a method for the measurement of hydroxyproline density distributions, as an estimate for collagen density distributions, in fibrous tissues such as ligments and tendons. To evaluate this method, a single flexor tendon of a human hand was divided into seven tissue locations. Triplicate determinations of the dry weight tissue mass, volume, and hydroxyproline mass were made at each location: two samples were analyzed at the same time (a and b) and one was analyzed later (c). The intralocation variation is an estimate for the measurement error variance, which indicates both the precision (a compared with b) and the repeatability (b compared with c) of the technique for determination of volume, dry weight tissue mass, hydroxyproline concentration, and hydroxyproline density. The precision was about 5% for all variables, and the repeatability ranged from 1.5–4.3%. In comparison with the interlocation variations, the error variances were small, except for collagen concentration. This indicates that despite the measurement errors, differences in hydroxyproline density can be detected within fibrous tissues with the proposed method. The use of only a single tendon is adequate to evaluate the measurement error of the method, but more tendons should be measured to generalize the absolute values of the variables.

Nonuniform distribution of collagen density in human knee ligaments

“…If the conversion factor is constant for human ligamentous tissues, relative differences in hydroxyproline density provide an estimate for relative differences in collagen density. The overall precision of the method is about 5% for the parameters V,, H,, and HJV, (11). The data on hydroxyproline density of the samples were grouped along each ligament bundle into proximal, proximal central, distal central, and distal segments (Fig.…”

The optimal starting time of postoperative intraperitoneal mitomycin-C therapy with preserved intestinal wound healing

“…No difference was observed between the controls and the other MTC sub-groups. Collagen synthesis, which may be reflected by tissue hydroxyproline content, is an essential feature of anastomotic healing in the intestine [17,26]. We therefore measured the tissue hydroxyproline content to determine whether MTC is detrimental to anastomotic wound healing.…”

A dynamic model of simulating stress distribution in the distal femur after total knee replacement

“…Halloran et al [14] developed an explicit FE TKR model that incorporated both the tibiofemoral and the patellofemoral articulations. In all these investigations of dynamic models, more attention has been paid to the tibiofemoral contact mechanics [9][10][11].…”