Method performance and multi-laboratory assessment of a normal phase high pressure liquid chromatography–fluorescence detection method for the quantitation of flavanols and procyanidins in cocoa and chocolate containing samples

Robbins, Rebecca J.; Leonczak, Jadwiga; Johnson, Judith; Li, Julia; Kwik‐Uribe, Catherine; Prior, Ronald L.; Gu, Liwei

doi:10.1016/j.chroma.2009.04.006

Cited by 118 publications

(138 citation statements)

References 22 publications

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“…Robbins et al [18] compared the separation efficiency of different brands of columns with similar packing material and found the Develosil diol phase column yielded the best chromatograms. Thus, …”

Section: Proanthocyanidins In Fresh Cranberry Extract Vs Commercial mentioning

confidence: 99%

Comparison of A-type Proanthocyanidins in Cranberry and Peanut Skin Extracts Using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Ye¹,

Neilson²,

Sarnoski³

et al. 2016

J Mol Genet Med

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Section: Proanthocyanidins In Fresh Cranberry Extract Vs Commercial mentioning

confidence: 99%

Comparison of A-type Proanthocyanidins in Cranberry and Peanut Skin Extracts Using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Ye¹,

Neilson²,

Sarnoski³

et al. 2016

J Mol Genet Med

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“…They are the phenolic compounds more abundant in cocoa and have a range of positive physiological effects in humans; most notably, they reduce blood pressure and reduce the risk of cardiovascular disease and stroke (Sudano et al 2012). Due to their structural diversity and complexity, the analytical methodology employed for the analysis of these compounds is based mainly upon DP instead of separation of individual compounds and specific isomers (Robbins et al 2009). Therefore, a normal-phase HPLC-FLD method using a HILIC column was carried out to accomplish the analysis of procyanidins with different degrees of polymerization (Robbins et al 2009;Robbins et al 2013;Robbins et al 2012).…”

Section: Determination Of Procyanidins By Hplc-fldmentioning

confidence: 99%

“…Due to their structural diversity and complexity, the analytical methodology employed for the analysis of these compounds is based mainly upon DP instead of separation of individual compounds and specific isomers (Robbins et al 2009). Therefore, a normal-phase HPLC-FLD method using a HILIC column was carried out to accomplish the analysis of procyanidins with different degrees of polymerization (Robbins et al 2009;Robbins et al 2013;Robbins et al 2012). This method allowed the measurement of the large structural diversity of these compounds by oligomeric size.…”

Section: Determination Of Procyanidins By Hplc-fldmentioning

confidence: 99%

“…The separation of flavanol oligomers was performed as previously described (Robbins et al 2009) using an Agilent 1100 series HPLC system (Agilent Technologies, Palo Alto, CA, USA) equipped with an autosampler, solvent degasser, quaternary pump, column heater, and DAD and fluorescence detectors. A Develosil Diol 100-Å column (250 × 4.6 mm; 100-Å pore size; 5-μm particle size; 35°C) from Phenomenex (Torrance, CA, USA) was used for separation.…”

Section: Analysis Of Procyanidins By Hplc With Fluorescence Detectormentioning

confidence: 99%

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Green and Efficient Extraction Method to Determine Polyphenols in Cocoa and Cocoa Products

et al. 2017

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Cocoa and cocoa products have broad and wellproven health benefits, most of which are associated with the high antioxidant capacity of cocoa flavonoids. Most extraction methods for flavonoids in cocoa products use several steps including a defatting step and large amount of organic solvents. Moreover, they are labor-intensive and time-consuming. In this work, a pressurized hot water extraction (PHWE) method has been compared to conventional sonication extraction (CSE) method. The contribution of individual compounds to the total antioxidant capacity of the extracts was evaluated by developing an analytical technique consisting of high-performance liquid chromatography (HPLC) with photodiode array coupled to electrochemical and to charged aerosol detectors and HPLCmass spectrometry for the confirmation of the identity of compounds present. Additionally, procyanidins were analyzed by fluorescence detection. PHWE turned out to be more efficient in extracting phenolics and methylxanthines, as compared to the conventional method, in addition to being more Bgreenî n terms of using less organic solvents.

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“…PACs identification analyses were determined according to Robbins et al (2009). Separation was carried out with a Waters Aquity UPLC system equipped with fluorescence detectors.…”

Section: Instrumentation and Chromatographic Conditions For Ellagitanmentioning

confidence: 99%

Characterization and effect of clarified araçá (Psidium guineenses Sw.) juice on postprandial glycemia in healthy subjects

Balisteiro¹,

Alezandro²,

Genovese³

2013

Ciênc. Tecnol. Aliment.

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Method performance and multi-laboratory assessment of a normal phase high pressure liquid chromatography–fluorescence detection method for the quantitation of flavanols and procyanidins in cocoa and chocolate containing samples

Cited by 118 publications

References 22 publications

Comparison of A-type Proanthocyanidins in Cranberry and Peanut Skin Extracts Using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Comparison of A-type Proanthocyanidins in Cranberry and Peanut Skin Extracts Using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Green and Efficient Extraction Method to Determine Polyphenols in Cocoa and Cocoa Products

Characterization and effect of clarified araçá (Psidium guineenses Sw.) juice on postprandial glycemia in healthy subjects

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