Method for the isolation and detection of Enterobacter sakazakii (Cronobacter) from powdered infant formula

Species of Cronobacter – A review of recent advances in the genus and their significance in infant formula milk

“…Alternative methods have subsequently been developed for the detection and enumeration of members of the genus Cronobacter (Druggan & Iversen, 2009;Iversen et al, 2004b;Lampel & Chen, 2009). The pre-enrichment step included in these methods is reported to recover injured cells and increase the sensitivity of the detection method.…”

“…The FDA is currently in the process of validating a new detection protocol for Cronobacter that will subsequently be adopted into the FDA's Bacteriological Analytical Manual (Druggan & Iversen, 2009;Lampel & Chen, 2009). The proposed protocol includes incubation at 36 AE 1 C in buffered peptone water after which the culture is streaked on DFI and R&F agar (R&F Laboratories, Downers Grove, IL: http://wwwrf-labs.com/).…”

Electrochemical immunosensor for Enterobacter sakazakii detection based on electrochemically reduced graphene oxide–gold nanoparticle/ionic liquid modified electrode

“…Recently, several molecular-based techniques for sensitive detection of E. sakazakii have been reported [2][3][4][5]. However, these molecular methods are costly and very complicated in sample pretreatment and measuring procedures.…”

Rapid detection of genus Cronobacter in powdered infant formula milk

“…are considered emerging foodborne pathogens that have been known to cause serious infections in infants, mainly neonates. Although the foodborne infections caused by these pathogens are not common, these pathogens have a 40-80% fatality rate in infected infants [3,4].…”

“…Thus, it is necessary to look for rapid, simple and reliable methods for detection of this pathogen. Several rapid techniques were developed [3], including mass spectrometry [15], and immunoassay [16]. The newly developed procedures seem to fulfil the criteria, but still have various shortcomings.…”