Method for the determination of five toxicologically relevant arsenic species in human urine by liquid chromatography–hydride generation atomic absorption spectrometry

Complementary chromatography separation combined with hydride generation–inductively coupled plasma mass spectrometry for arsenic speciation in human urine

“…The most common arsenic species found in human urine are dimethylarsinic acid (DMA(V)), monomethylarsonic acid (MMA(V)), arsenite (As(III)), and arsenate (As(V)) [6][7][8][9][10][11][12]. The toxicity of arsenic varies with different chemical forms, e.g., arsenobetaine (AsB) is essentially a nontoxic form of arsenic [11][12][13][14][15][16][17][18].…”

“…The toxicity of arsenic varies with different chemical forms, e.g., arsenobetaine (AsB) is essentially a nontoxic form of arsenic [11][12][13][14][15][16][17][18]. If an individual consumes seafood, e.g., crab and lobster, within three days prior to the collection of urine sample, then AsB can be the dominating arsenic specie in urine [10,13]. This presence of AsB in urine along with As(III), As(V), MMA(V), and DMA(V) renders the measurement of total urinary arsenic not suitable for assessing human exposure to toxic forms of arsenic.…”

Application of Organometallic Speciation in Clinical Studies

“…An online coupling method for the simultaneous quantification of As(III), As(V), MMA(V), DMA(V), and TMAO in human urine by LC-HG-AAS was presented by Sur and co-workers [71]. After separation by AEC, the As species were reduced online to their corresponding hydrides by an HG system and detected by AAS.…”

“…NaBH 4 solution for reduction of the As species to AsH 3 (0.5% in 0.05% aqueous NaOH, w/v) was pumped into the system. The method has been applied to determine As species in the urine of a volunteer before and after consumption of seafood as well as to analyze certified reference materials (CRMs) in urine matrix for their As species content [71].…”

Arsenic exposure assessment of children living in a lead mining area in Southeastern Brazil

“…Several analytical techniques may be used for speciation studies, such as high performance liquid chromatography (HPLC) coupled with flow injection-hydride generation atomic absorption spectrometry (HG AAS) 15,16 , or inductively coupled plasma mass spectrometry detection (ICP MS) 17 . Guo et al 18 have proposed methods for detecting only the toxicological relevant species using cystein and HG AAS.…”