Method for the concentration of infectious pancreatic necrosis virus from hatchery water

Maheshkumar, S.; Goyal, Sagar M.; Peterson, Richard B.; Economon, Philip P.

doi:10.1016/0166-0934(91)90159-w

Cited by 13 publications

(3 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The water supply for a hatchery can harbour fish and invertebrates that are carriers of the IPNV. The introduction of free virus via the water supply is thought to occur, although no one has yet isolated IPNV from open or closed water supplies (Maheshkumar et al., 1991 ; Bebak et al., 1998 ). Predatory birds can transfer infected fish between raceways and from water supplies outside of a hatchery and IPNV has also been imported on bird faeces (McAllister and Owens, 1992 ) and bird bills, beaks, feathers and legs (Peters and Neukirch, 1986 ; Smith et al., 2000 ).…”

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infectious pancreatic necrosis (IPN)

Nielsen¹,

Álvarez²,

Calistri³

et al. 2023

EFS2

View full text Add to dashboard Cite

Infectious pancreatic necrosis (IPN) was assessed according to the criteria of the Animal Health Law (AHL), in particular, the criteria of Article 7 on disease profile and impacts, Article 5 on its eligibility to be listed, Annex IV for its categorisation according to disease prevention and control rules as in Article 9, and Article 8 for listing animal species related to IPN. The assessment was performed following a methodology previously published. The outcome reported is the median of the probability ranges provided by the experts, which indicates whether each criterion is fulfilled (lower bound ≥ 66%) or not (upper bound ≤ 33%), or whether there is uncertainty about fulfilment. Reasoning points are reported for criteria with an uncertain outcome. According to the assessment here performed, it is uncertain whether IPN can be considered eligible to be listed for Union intervention according to Article 5 of the AHL (50-90% probability). According to the criteria in Annex IV, for the purpose of categorisation related to the level of prevention and control as in Article 9 of the AHL, the AHAW Panel concluded that IPN does not meet the criteria in Section 1 (Category A; 0-1% probability of meeting the criteria) and it is uncertain whether it meets the criteria in Sections 2, 3, 4 and 5 (Categories B, C, D and E; 33-66%, 33-66%, 50-90% and 50-99% probability of meeting the criteria, respectively). The animal species to be listed for IPN according to Article 8 criteria are provided.

show abstract

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infectious pancreatic necrosis (IPN)

Nielsen¹,

Álvarez²,

Calistri³

et al. 2023

EFS2

View full text Add to dashboard Cite

show abstract

“…Se han descrito métodos de extracción en polímeros de dos fases (Cohen 1973, Tu 1974, Grinnel y Leong 1979, metodologías basadas en la adsorción-elusión del virus (Eliasson y col., 2003b) y procedimientos de ultrafiltración, tanto sobre filtros de membrana cargados positivamente (Maheshkumar y col 1991a, 1991b, 1992, McAllister y Bebak 1997 como mediante la utilización de la ultrafiltración de flujo tangencial (UFT) (Watanabe 1988).…”

Section: Introduccionunclassified

Concentración del virus de la necrosis pancreática infecciosa mediante ultrafiltración de flujo tangencial combinado con filtración de exclusión

et al. 2006

View full text Add to dashboard Cite

SUMMARYThe infectious pancreatic necrosis virus, IPNV, is the etiological agent of a highly contagious disease that affects young salmon. The virus is mostly horizontally transmitted; therefore virus quantitation in water is mandatory for a proper sanitary management in the salmon industry as well as in the environment close to the hatcheries. To evaluate the water as a risk factor for IPNV infection in cultured or wild animals, it is necessary to titrate the virus with a suitable sensitivity. Therefore, a methodology that allows IPNV concentration to be determined in water using two kinds of ultrafiltration was developed. One, based on the tangential flow of the sample along the surface of a membrane, the other based on the retention of the viral particles at the surface of membranes with controlled pore diameter. Reagents and conditions were selected to reduce virus inactivation and/or virus binding to the filters. Finally, a protocol was developed that allows the concentration of IPNV in three orders of magnitude and with a total recovery of infectivity to be determined. This method can be performed in less than one day including virus titration using the fluorescent foci method INTRODUCCIONEl virus de la necrosis pancreática infecciosa (virus IPN) es considerado como el virus de especies salmonídeas más prevalente en el mundo. Es el agente responsable de la necrosis pancreática infecciosa (IPN), enfermedad que es una de las principales patologías a las que se ha visto enfrentada la salmonicultura nacional y mundial en los últimos 10 años. Según datos recientes, los brotes del virus IPN en el país afectaron al 48% del total de las pisciculturas, al 61% de los centros de esmoltificación y al 49% de los centros de engorda en 1999 (FIP 2003) provocando enormes pérdidas al sector.El virus IPN causa una infección sistémica aguda en alevines de 1 a 6 meses de edad, pudiendo provocar, incluso, mortalidades acumulativas del orden del 100% de la población afectada. Los peces sobrevivientes permanecen en estado portador de por vida, diseminando el virus por largo tiempo, manteniendo así el virus en el agua (Wolf 1988). Sólo la detección del virus activo causante de la necrosis pancreática infecciosa puede indicar el verdadero nivel de riesgo para una piscicultura. Las técnicas empleadas con este propósito suelen ser lentas en su aplicación, sin embargo, recientemente se desarrolló una téc-nica que permite titular al virus IPN en menos de 20 h. La metodología se basa en la visualización mediante microscopia de epifluorescencia (MEF) de la amplificación del virus en células CHSE-214. Las células infectadas (fluorescencia positiva) son cuantificadas como Focos Fluorescentes (FF) de forma equivalente a la técnica de unidades formadoras de placas (Espinoza y Kuznar 2002).Para desarrollar la enfermedad un animal debe estar expuesto a una concentración mínima de virus; en principio, para el virus IPN, ésta debiera ser sobre 1x10 2 UFP/l

show abstract

“…A weighed aliquot of the pH 5.5 water (about 5 kg) was passed through a filter array consisting of a glass prefilter (1.0 µm Viral infectivity assays of fish tissue average-pore-diameter) and two 47-mm electropositive virosorb 1-MDS filters (Maheshkumar, Salmonid and non-salmonid fish were collected by electrofishing and held at about 4°C. The fish were Goyal & Economon 1991a; Maheshkumar, Goyal, Peterson & Economon 1991b). The sample was assayed for virus within 24 h of capture.…”

Section: Introductionmentioning

confidence: 99%

Infectious pancreatic necrosis virus in the environment: relationship to effluent from aquaculture facilities

McAllister

Bebak

1997

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

Method for the concentration of infectious pancreatic necrosis virus from hatchery water

Cited by 13 publications

References 12 publications

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infectious pancreatic necrosis (IPN)

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infectious pancreatic necrosis (IPN)

Concentración del virus de la necrosis pancreática infecciosa mediante ultrafiltración de flujo tangencial combinado con filtración de exclusión

Infectious pancreatic necrosis virus in the environment: relationship to effluent from aquaculture facilities

Contact Info

Product

Resources

About