Method for regeneration of hormones: 17β-estradiol, 21α-hydroxyprogesterone and corticosterone. A pathway for a possible medical application

Abstract

The hormones 17β-estradiol (17βE2), 21α-hydroxyprogesterone (21α-HOPRG) and corticosterone (CORT) were used as representative models for the study. As a source for hormone excitation in singlet state serviced monochromatic UV-light (λ=254 nm), it was stated that the transients resulting by e-aq emission in air-free mixture water/ethanol 40/60, as long as they are in "status nascendi", can be regenerated by electron transfer from a potent electron donor, e.g., vitamin C. The hormone regeneration (%) strongly depends, after all, on specific hormone molecular structure, concentration, temperature, etc. Because of the large heterogenic molecular structures, the substrates dissolved in the solvent mixture form "associates" (unstable complexes) in concentrations >109 mol/L hormone. The hormones eject, but they also consume e-aq with a rather high reaction rate constant (k≈109 up to 2×1010 L/mol.s), therefore, they act as "electron mediators". It was also observed that the hormones by dissolution in aerated solvent mixture are sensitive towards oxygen. For an explanation of the results, probable reaction mechanisms are presented. The described method offers a new pathway and possibilities for application in medicine.

Hormones: electron emission, communication, mutual interaction, regeneration, metabolites, carcinogenesis and receptor action

“…This effect is attributed mainly to the aromatic structure of ring A and to the OH-groups, particularly in their dissociated state [6] . Estrogen hormones form similar ' associates ' at higher concentrations ( > 10 −7 mol/L) [8,9] . This effect can be monitored in two ways: spectroscopically, as mentioned before, but also by decreasing Q(e aq − )-yield with increasing hormone concentration ( Table 3 ).…”

“…Firstly, I will discuss the reaction mechanisms of the e aq − emission from estrogens, having an aromatic ring A of the molecule. Taking 17 β -estradiol (17 β E2) as a representative, the following reactions can take place when it is electronically excited in water/ethanol = 40/60 media [8,9,15,16] . Based on previous studies [6] , the formation of the hormone radicals R 1…”

“…. 9 e aq -Similar to PRG (Eq. 11), TES • + species can also be regenerated by reaction with H 2 O, at least partly.…”

“…Based on the similarity of the molecular structure, the mechanisms of e aq − emission from 17 α -OHPR, 21 α -OHPRG and corticosterone (CORT) is very likely to those of PRG and TES discussed above [8,9,11,17,18] . A radical cation (R • + ) is formed in all cases, because a part of ejected e aq − results from ring A of the corresponding hormone.…”

“…The hormone regeneration was achieved by electron transfer from a potent electron donor-like vitamin C (Vit C) during the life time (status nascendistate) of the hormone transients. The regeneration effect was reported of progesterone (PRG), testosterone (TES) and estrone (E1) [23] as well as of 17 β -estradiol (17 β E2), 21 α -hydroxyprogesterone (12 α -OHPRG) and corticosterone (CORT) [9] in polar media (40/60 = water/ethanol), pH = 7.4 and 37 ° C by substrate excitation with UV-light ( λ = 254 nm). In order to avoid hormone oxidation, the samples were dissolved and UV-irradiated in airfree media.…”