Method for measuring nitrate-nitrogen in vegetables using anion-exchange high-performance liquid chromatography

Hunt, John W.; Seymour, Deborah J.

doi:10.1039/an9851000131

Cited by 34 publications

(9 citation statements)

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“…On receipt at the laboratory, samples were stored at 48C until they were prepared in accordance with normal domestic practice (but not cooked) before being freeze-dried, milled and stored frozen until analysis. Samples were analysed by a method based on that of Hunt and Seymour (1985). Nitrate was extracted using water and interfering compounds removed using activated carbon.…”

Section: Materials and Methods Smentioning

confidence: 99%

Dietary exposures to nitrate in the UK

Ysart¹,

Miller²,

Barrett³

et al. 1999

Food Additives and Contaminants

100

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Dietary exposures to nitrate have been estimated for two different groups of UK consumers. The daily dietary exposure of the general population was estimated from the UK 1997 Total Diet study to be 52 mg/day. Vegetables contributed approximately 70% to this total dietary exposure. Dietary exposure estimates for adult consumers of vegetables commonly eaten in the UK were 93 mg/day and 140 mg/day for mean and 97.5th percentile consumers, respectively. Dietary exposures to nitrate of UK consumers are similar to those in other European countries and are less than the Acceptable Daily Intake (ADI) for nitrate set by the European Commission's Scientific Committee for Food.

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Section: Materials and Methods Smentioning

confidence: 99%

Dietary exposures to nitrate in the UK

Ysart¹,

Miller²,

Barrett³

et al. 1999

Food Additives and Contaminants

100

View full text Add to dashboard Cite

show abstract

“…Concentrations of organic N were determined by the indophenol blue method (Crooke and Simpson, 1971). Nitrate N concentrations were measured on water extracts of the plant dry matter (50ml::100mg) using HPLC (Hunt and Seymour, 1985). Total N concentrations were calculated from the sum of the corresponding organic and nitrate N concentrations.…”

Section: Growth Measurements and Chemical Analysismentioning

confidence: 99%

Influence of plant nutrient concentration on growth rate: Use of a nutrient interruption technique to determine critical concentrations of N, P and K in young plants

Burns¹

1992

Plant Soil

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A method is described for determining the way in which growth rate varies with plant nutrient concentration using a simple nutrient interruption technique incorporating only 2 treatments. The method involves measuring the changes in growth and nutrient composition of otherwise well-nourished plants after the supply of one particular nutrient has been withheld. Critical concentrations are estimated from the relationship between the growth rate (expressed as a fraction of that for control plants of the same size which remained well-nourished throughout) and the concentration of the growth-limiting nutrient in the plants as deficiency developed. Trials of the method using young lettuce plants showed that shoot growth rate was directly proportional to total N (nitrate plus organic N) concentration, and linearly or near-linearly related to K and P concentration over a wide range; the corresponding relationship for nitrate was strongly curvi-linear. Critical concentrations (corresponding to a 10% reduction in growth rate) determined from these results were similar to critical values calculated from models derived from field data, but were generally higher than published estimates of critical concentration (based on reductions in shoot weight) for plants of a similar size. Reasons for these discrepancies are discussed. Nitrate, phosphate or potassium concentrations in sap from individual leaf petioles were highly sensitive to changes in shoot growth rate as deficiency developed, with the slope of the relationships varying with leaf position, due to differences both in their initial concentration and in the rates at which they were utilized in individual leaves. Each nutrient was always depleted more quickly in younger leaves than in older ones, providing earlier evidence of deficiency for diagnostic purposes. Although the plants were capable of accumulating nitrate, phosphate and potassium well in excess of that needed for optimum dry matter production during periods of adequate supply, the rate of mobilization of these reserves was insufficient to prevent reductions in growth rate as the plants became deficient. This brings into question the validity of the conventional concept that luxury consumption provides a store of nutrients which are freely available for use in times of shortage. The implications of these results for the use of plant analysis for assessing plant nutrient status are discussed.

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“…All ion fluxes were related to fresh weight of the root tissue used during uptake studies. Nitrate ions in these samples were measured by highperformance liquid chromatography using 10 mm×250 mm analytical anion exchange column (Whatman Partisil-10 SAX), according to Hunt and Seymour (Hunt and Seymour 1985). UV detector was used at 210 nm.…”

Section: Biomass Accumulation and Nitrogen Efficiencymentioning

confidence: 99%

Nitrogen-efficient rice cultivars can reduce nitrate pollution

Hakeem

Ahmad

Iqbal

et al. 2011

Environ Sci Pollut Res

102

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This study suggests that the HATS, high NR, and glutamine synthetase activity and the soluble protein content distribution have a key role in N efficiency of rice genotypes. These parameters may be considered in breeding and genetic engineering programs for improving the NUE of rice, which might be helpful in reducing the fertilizer loss, hence decreasing environmental degradation and improving crop productivity through improvement of nitrogen utilization efficiency in rice.

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Method for measuring nitrate-nitrogen in vegetables using anion-exchange high-performance liquid chromatography

Cited by 34 publications

References 0 publications

Dietary exposures to nitrate in the UK

Dietary exposures to nitrate in the UK

Influence of plant nutrient concentration on growth rate: Use of a nutrient interruption technique to determine critical concentrations of N, P and K in young plants

Nitrogen-efficient rice cultivars can reduce nitrate pollution

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