Method for Isolating Auxotrophic Mutants in Aspergillus nidulans Using N-glycosyl-polifungin

Bal, Jerzy; Balbin, Ewa; Pieniacżek, N. J.

doi:10.1099/00221287-84-1-111

Cited by 10 publications

(1 citation statement)

References 11 publications

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“…The survival of C. obtusa and Z. fermentati obtained after nitrosoguanidine treatment (27 and 20%, respectively) was similar to that already described for S. cerevisiae (12). However, survival to polifungin treatment (2 to 3%), as well as the frequency of auxotroph mutants that were obtained (less than 0.1%), was significantly different from those reported for A. nidulans (6) and, above all, S. cerevisiae (36). Since this antibiotic acts on the membrane lipids, mainly sterols (47), it might be that lipid composition in these yeasts is very different from that of S. cerevisiae or A. nidulans.…”

Section: Methodscontrasting

confidence: 68%

Intergeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion

Piña¹,

Calderón²,

Benı́tez³

1986

Appl Environ Microbiol

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To obtain strains that are able to efficiently produce ethanol from different carbohydrates, mainly cellulose hydrolysates, several species of the genus Candida and a Zygosaccharomyces fermentati strain were examined for their ability to utilize cellobiose and produce ethanol, as well as for their thermotolerance and the possibility of genetic manipulation. Candida obtusa and Zygosaccharomycesfermentati tolerated the maximal temperature for growth, possessed the highest cellobiase activity, and offered the possibility of genetic manipulation, although neither of them proved to be a good producer of ethanol. Intergeneric hybrids of Saccharomyces cerevisiae and Z. fermentati were obtained after protoplast fusion. They were selected as prototrophic strains, after isolation of auxotrophic mutants from Z. fermentati and fusion with an S. cerevisiae strain which was also auxotrophic. The hybrids, which appeaired at a frequency of 2 x 10-7, presented characteristics of both parents, such as resistance to certain drugs and the ability to grow with either cellobiose or lactic acid as the sole carbon source; they were very stable, even under nonselective conditions. These hybrids may have important industrial applications as good fermenting strains.

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Section: Methodscontrasting

confidence: 68%