Method for intracellular magnetic labeling of human mononuclear cells using approved iron contrast agents

Sipe, Jack C.; Filippi, Massimo; Martino, Gianvito; Furlan, Roberto; Rocca, Maria A.; Rovaris, Marco; Bergami, A.; Zyroff, Jack; Scotti, Giuseppe; Comi, Giancarlo

doi:10.1016/s0730-725x(99)00085-5

Cited by 61 publications

(31 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By use of optimised radio-frequency coils, specialised magnetic gradients (Heyn et al 2006), long scan times (2 h), and high magnetic Welds [Hoehn et al (2002): 7 T; Kircher et al (2003): 8.5 T], adequate signal-to-noise ratios can be obtained, allowing detection of approximately single cells loaded with nanoparticulate iron oxides (ultra-small particles of iron oxide, "USPIOs", 30 nm diameter); indeed, single cells can even be detected by use of a 1.5 T clinical scanner (Foster-Gareau et al 2003). A signiWcant advance was the realisation (Engberink et al 2007) that the iron oxide particles loaded into the cells need not be nanoparticles (Benderbous et al 1996;Wang et al 2001;Jung 1995) but that better results could be obtained with larger nanoparticles (small particles of iron oxide, "SPIOs", 150 nm diameter) (Zelivyanskaya et al 2003;Sipe et al 1999;Metz et al 2004), and that excellent results can be obtained by loading the cells with micrometre-sized iron oxide particles (micro-particles of iron oxide, "MPIOs") (Shapiro et al 2005;Hinds et al 2003;Wu et al 2006). A basic problem of iron particles is that they cause a signal void (negative contrast), which is diYcult to quantify.…”

Section: Why Nanoparticles?mentioning

confidence: 99%

Molecular imaging with nanoparticles: giant roles for dwarf actors

Debbage

Jaschke

2008

Histochem Cell Biol

233

212

View full text Add to dashboard Cite

Molecular imaging, first developed to localise antigens in light microscopy, now encompasses all imaging modalities including those used in clinical care: optical imaging, nuclear medical imaging, ultrasound imaging, CT, MRI, and photoacoustic imaging. Molecular imaging always requires accumulation of contrast agent in the target site, often achieved most efficiently by steering nanoparticles containing contrast agent into the target. This entails accessing target molecules hidden behind tissue barriers, necessitating the use of targeting groups. For imaging modalities with low sensitivity, nanoparticles bearing multiple contrast groups provide signal amplification. The same nanoparticles can in principle deliver both contrast medium and drug, allowing monitoring of biodistribution and therapeutic activity simultaneously (theranostics). Nanoparticles with multiple bioadhesive sites for target recognition and binding will be larger than 20 nm diameter. They share functionalities with many subcellular organelles (ribosomes, proteasomes, ion channels, and transport vesicles) and are of similar sizes. The materials used to synthesise nanoparticles include natural proteins and polymers, artificial polymers, dendrimers, fullerenes and other carbon-based structures, lipid-water micelles, viral capsids, metals, metal oxides, and ceramics. Signal generators incorporated into nanoparticles include iron oxide, gadolinium, fluorine, iodine, bismuth, radionuclides, quantum dots, and metal nanoclusters. Diagnostic imaging applications, now appearing, include sentinal node localisation and stem cell tracking.

show abstract

Section: Why Nanoparticles?mentioning

confidence: 99%

Molecular imaging with nanoparticles: giant roles for dwarf actors

Debbage

Jaschke

2008

Histochem Cell Biol

233

212

View full text Add to dashboard Cite

show abstract

“…This increased harvest in enhancing lesion detection is likely due to the visualization of areas with "low-grade" inflammation. Progress has also been made in specific cell labeling by taking advantage of the ability of professional phagocytes (macrophages and activated resident microglia) to internalize ad hoc MRI contrast agents [18]. As a consequence, MS lesions containing this cell type can be detected.…”

Section: Measuring Inflammationmentioning

confidence: 99%

Inflammatory demyelination and neurodegeneration in early multiple sclerosis

Charil

Filippi

2007

Journal of the Neurological Sciences

View full text Add to dashboard Cite

“…Consequently, these contrast agents are not used as isolated reagents to label hNSCs or other mammalian cells [20][21][22]. In most cases, internalization of nanoparticles by hNSCs requires the use of transfection agents (TAs), like protamine sulfate (PS) or poly-L-lysine (PL) to achieve an efficient labeling of the stem cells.…”

Section: Introductionmentioning

confidence: 99%

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Ramos-Gómez¹,

Seiz²,

Martínez‐Serrano³

2016

Nano Online

View full text Add to dashboard Cite

Background: Magnetic resonance imaging is the ideal modality for non-invasive in vivo cell tracking allowing for longitudinal studies over time. Cells labeled with superparamagnetic iron oxide nanoparticles have been shown to induce sufficient contrast for in vivo magnetic resonance imaging enabling the in vivo analysis of the final location of the transplanted cells. For magnetic nanoparticles to be useful, a high internalization efficiency of the particles is required without compromising cell function, as well as validation of the magnetic nanoparticles behaviour inside the cells. Results: In this work, we report the development, optimization and validation of an efficient procedure to label human neural stem cells with commercial nanoparticles in the absence of transfection agents. Magnetic nanoparticles used here do not affect cell viability, cell morphology, cell differentiation or cell cycle dynamics. Moreover, human neural stem cells progeny labeled with magnetic nanoparticles are easily and non-invasively detected long time after transplantation in a rat model of Parkinson's disease (up to 5 months post-grafting) by magnetic resonance imaging. Conclusions: These findings support the use of commercial MNPs to track cells for short-and mid-term periods after transplantation for studies of brain cell replacement therapy. Nevertheless, long-term MR images should be interpreted with caution due to the possibility that some MNPs may be expelled from the transplanted cells and internalized by host microglial cells.

show abstract

Method for intracellular magnetic labeling of human mononuclear cells using approved iron contrast agents

Cited by 61 publications

References 7 publications

Molecular imaging with nanoparticles: giant roles for dwarf actors

Molecular imaging with nanoparticles: giant roles for dwarf actors

Inflammatory demyelination and neurodegeneration in early multiple sclerosis

Optimization of the magnetic labeling of human neural stem cells and MRI visualization in the hemiparkinsonian rat brain

Contact Info

Product

Resources

About