Method for Determination of Intracellular Sodium in Perfused Cancer Cells by 23Na Nuclear Magnetic Resonance Spectroscopy

Hansen, Lothar L.; Rasmussen, J.; Friche, Ellen; Jaroszewski, Jerzy W.

doi:10.1006/abio.1993.1530

Cited by 6 publications

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“…Various experimental approaches have been developed to study changes in [Na + ] i . For example, 23 Na nuclear magnetic resonance spectroscopy offers a reliable method for non-invasive quantification of [Na + ] i in perfused cancer cells and tissues (Hansen et al 1993 ). Flow cytometry also provides feasible [Na + ] i measurements (Amorino and Fox 1995 ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

et al. 2016

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Sodium (Na+) ions are known to regulate many signaling pathways involved in both physiological and pathological conditions. In particular, alterations in intracellular concentrations of Na+ and corresponding changes in membrane potential are known to be major actors of cancer progression to metastatic phenotype. Though the functionality of Na+ channels and the corresponding Na+ currents can be investigated using the patch-clamp technique, the latter is rather invasive and a technically difficult method to study intracellular Na+ transients compared to Na+ fluorescence imaging. Despite the fact that Na+ signaling is considered an important controller of cancer progression, only few data using Na+ imaging approaches are available so far, suggesting the persisting challenge within the scientific community. In this study, we describe in detail the approach for application of Na+ imaging technique to measure intracellular Na+ variations in human prostate cancer cells. Accordingly, we used three Na+-specific fluorescent dyes–Na+-binding benzofuran isophthalate (SBFI), CoroNa™ Green (Corona) and Asante NaTRIUM Green-2 (ANG-2). These dyes have been assessed for optimal loading conditions, dissociation constant and working range after different calibration methods, and intracellular Na+ sensitivity, in order to determine which probe can be considered as the most reliable to visualize Na+ fluctuations in vitro.

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Section: Introductionmentioning

confidence: 99%

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

et al. 2016

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“…Na + is actively pumped from the intracellular to the extracellular space to maintain a [Na + ] gradient on the order of a factor of 10, with intracellular Na + concentrations, [Na + ] i , usually attaining 10–15 mM. Therefore, methods for in vivo measurement of [Na + ] i in biological tissue have been proposed in recent years, notably MRS techniques based on the chemical‐shift effects of [Na + ] i on 19 F MRS resonances of FCrown‐1 (a fluorinated tribenzo‐diaza‐18‐crown‐6 compound) or FCryp‐1 (another fluorinated tribenzo crown ether), but also 23 Na MRS methods based on chemical‐shift reagents such as dysprosium or thulium complexes (see for further chemical details regarding FCrown‐1 and FCryp‐1). However, these methods have not taken into consideration [Na + ] i heterogeneity within a measured volume or voxel.…”

Section: Introductionmentioning

confidence: 99%

A method for multiparametric statistical quantification of the heterogeneity of free Na⁺concentration by¹⁹F MR spectroscopy: Proof of principlein silicoandin vitro

Lutz

Bernard

2019

NMR in Biomedicine

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Sodium(I) (Na+) is one of the most important cations in mammalian tissues. Since Na+ plays a key role in basic cell function, noninvasive methods for measuring intracellular concentrations of free sodium ions in biological tissue have been developed on the basis of 19F NMR spectroscopy. However, intracellular Na+ levels are often not uniform throughout a tissue volume (or voxel) being measured. In such cases, [Na+] heterogeneity is not reflected in results obtained by the classical technique, and may even result in biased average values. For this reason, we have designed an approach for quantifying [Na+] heterogeneity. First, the 19F MRS resonance from FCrown‐1 serving as a “Na+ probe” is transformed into a [Na+] curve. Then the digital points of the resulting [Na+] profile are used to construct a histogram with specially developed algorithms. From each [Na+] histogram, at least eight quantitative parameters describing the underlying statistical [Na+] distribution were computed: weighted median, weighted mean, standard deviation, range, mode(s), kurtosis, skewness, and entropy. In addition to our new paradigm, we present a first validation based on (i) computer simulations and (ii) experimentally obtained 19F MR spectra of model solutions. This basic proof of principle warrants future in vivo experiments, in particular because of its ability to provide quantitative information complementary to that made available by commonly used 23Na MRI: (i) multiparametric statistical characterization of [Na+] distributions; (ii) total [Na+] heterogeneity analysis not intrinsically limited by the size of any MRI voxels; and (iii) analysis of unequivocally intracellular [Na+], as opposed to measurement of a combination of intra‐ and extracellular [Na+].

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Effect of Gossypol on Cultured TM3 Leydig and TM4 Sertoli Cells:31P and23Na NMR Study

Hansen

Jaroszewski²

1996

NMR Biomed.

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Method for Determination of Intracellular Sodium in Perfused Cancer Cells by 23Na Nuclear Magnetic Resonance Spectroscopy

Cited by 6 publications

References 0 publications

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

A method for multiparametric statistical quantification of the heterogeneity of free Na⁺concentration by¹⁹F MR spectroscopy: Proof of principlein silicoandin vitro

Effect of Gossypol on Cultured TM3 Leydig and TM4 Sertoli Cells:31P and23Na NMR Study

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Method for Determination of Intracellular Sodium in Perfused Cancer Cells by 23Na Nuclear Magnetic Resonance Spectroscopy

Cited by 6 publications

References 0 publications

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

Comparison of fluorescence probes for intracellular sodium imaging in prostate cancer cell lines

A method for multiparametric statistical quantification of the heterogeneity of free Na+concentration by19F MR spectroscopy: Proof of principlein silicoandin vitro

Effect of Gossypol on Cultured TM3 Leydig and TM4 Sertoli Cells:31P and23Na NMR Study

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A method for multiparametric statistical quantification of the heterogeneity of free Na⁺concentration by¹⁹F MR spectroscopy: Proof of principlein silicoandin vitro