Abstract:The use of Concanavalin A as a coupling agent for sensitization of sheep erythrocytes with an alkaline extract of Neisseria gonorrhoeae organisms is described in this paper. The sensitized erythrocytes were used in a plaque assay for enumeration of antibody-producing cells and in hemagglutination and hemolysin tests for determination of serum antibody. Rabbits were immunized intraperitoneally, intravenously, or via the footpad with viable N. gonorrhoeae organisms. Plaque-forming cells were found in draining ly… Show more
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