Method for correcting molecular weight from gel‐permeation chromatography. I. Skewing and concentration corrections

Nakano, Seikou; Goto, Yukitaka

doi:10.1002/app.1975.070191002

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…A technique to correct for nonlinear calibration has been published (30). Skewing corrections have been dealt with by several investigators (19,135). One technique that corrects for imperfect resolution is said to allow the use of equipment with inherently poor resolution.…”

Section: Calibration Of Gpc the Universal Calibration Techniquementioning

confidence: 99%

Rubber

Wadelin¹,

Morris²

1977

Anal. Chem.

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Section: Calibration Of Gpc the Universal Calibration Techniquementioning

confidence: 99%

Rubber

Wadelin¹,

Morris²

1977

Anal. Chem.

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“…The solutions are unstable, and especially in the marginal parts of the chromatogram there are considerable oscillations in the course of the corrected functions [2].…”

Section: Introductionmentioning

confidence: 99%

Correction for axial dispersion of data obtained by gel permeation chromatography with an on‐line viscometer

Netopilı́k

Schulz²

1984

Acta Polymerica

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Correction to axial dispersion of the results of analyses of polystyrene samples using a gel permeation chromatograph with an on-line viscometer was carried out by a procedure suggested in a preceding paper. Data obtained with each of the detectors of the apparatus were corrected separately, instead of correction of the molecular weight values calculated using data of both detectors. In this case there is no need to use procedures based on the solution of systems of linear equations: any method can be used which has originally been suggested only for correction of the concentration record, which in our case is the method of PIERCE and ARMONAS. Die Korrektur der yon einem Gelpermeationschromatographen mil on-line-gekoppeltem Viskosimeter erhaltenen MePwerte hinsichtlich der axialen DispersionDie Korrektur der an Polystyrenproben mittels eines Gelpermeationschromatographen mit on-line-Viskosimeter erhaltenen Analysenergebnisse wurde unter Verwendung eines Verfahrens dnrchgefiihrt, das in einer vorausgegangenen hrbeit beschrieben wurde. Die von den beiden Detektoren gelieferten Werte werden getrennt korrigiert, anstelle einer Korrektur der Molekulmassen unter Verwendung der Angaben beider Detektoren. Damit besteht keine Notwendigkeit, Systeme von linearen Gleichungen zu losen, man kann vielmehr jede Methode anwenden, die zur Korrektur der Konzentrationskurve geeignet ist. I n dieser Arbeit ist es die Methode von PIERCE und ARMONAS. Ilonpasica na ocesoe paccesnue aannbtx zem-nponuiEamzyeiL xpowamozpaguu c nocJceaymqe6 sucicoauwempueiLn o n p a m a H a oceBoe paccemme p e 3 y n b~a r o~ ~H~J I H~O B 0 6 p a s s o~ nonucTupona H a xpoMaTorpa4e AJIH r e a m p o m -KaIOKI(er0 aHaJIH3a (mx) C nPHC0eAHHeHHHM BHCKOBHMeTPOM 6una CAeJIaHa C HCnOJIb30BaHHeM MeTOAa, ripen-JIOXeHHOrO B IIpe,QIIIeCTBylO~e8 pa6ore. nOIIpaBKy H e BHOCHJIM B 3HaqeHHR MOJIeKyJIfIpHbIX MaCC, BbI'IHCJIeHHbIe H a OCHOBaHHH AaHHbIX o6onx AeTeKTOpOB annapaTypbI, K a K 3 T 0 0 6~9~0 AeJIaeTCH, a B AaHHbIe KaH(AOr0 H3 ABYX AeTeKTOpOB annapaTypbI B OTAeJIbHOCTH. B TaHOM CJIysae HeT HeO6XOAHMOCTH nOJIb30BaTbCH MeTOAaMH, OCHOBaHHbIMH H a PeIIIeHHH CHCTeM JIHHefiHbIX ypaBHeHH8 H K a K IIpaBHJIO He AaIoKI(HMH XOPOIIIHX pe3yJIbTaTOB, HO M O X H O IICIIOJIb-30BaTb ~110608 MeTOR, IIepBOHaqaJIbHO pa3pa60TaHHbIfi TOJIbKO AJIR BHeCeHHFi IIOnpaBKE B 3anECb KOHqeHTpaqEE, B HameM cnyqaa MeTOA nBPCA H APMOHACA.1220 1180 665 190 Acta Polymerica 35 (1984) Nr. 2 143 M , = 1.35 x 10' (LS) M , = 1.19 x l o 6 (LS) M , = 8.78 : 105 (LS) M , = 8.31 x 104 (0s)Table 1. Unfractionated samples. M , was calculated using K =0.039, a = 0.58 1 2 3 4 132 130 93 45 Table 2. Results of GPC analyses. Data in column " M ' shows if the calibration dependence ("cal") or detectors data ("det") were used in the calculation; column "cor" indicates if the data M cal cal det det cal cal det det cal cal det det cal cal det det cal cal det det cor were corrected Sample No 1, c = 14.8 mg/ml

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“…However, most of the correction procedures based on the solution of a system of linear equations, often very involved [3], do not give good results in the correction of the chromatogram and completely fail if used in the calculation of corrected molecular weights. The solutions are unstable, and especially in the marginal parts of the chromatogram there are considerable oscillations in the course of the corrected functions [2].…”

Section: Introductionmentioning

confidence: 99%

Correction for axial dispersion in gel permeation chromatography with a detector of molecular masses

Netopilı́k

1982

Polymer Bulletin

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Correction to axial dispersion of the results of analyses of polystyrene samples using a gel permeation chromatograph with an on-line viscometer was carried out by a procedure suggested in a preceding paper. Data obtained with each of the detectors of the apparatus were corrected separately, instead of correction of the molecular weight values calculated using data of both detectors. In this case there is no need to use procedures based on the solution of systems of linear equations: any method can be used which has originally been suggested only for correction of the concentration record, which in our case is the method of PIERCE and ARMONAS. Die Korrektur der yon einem Gelpermeationschromatographen mil on-line-gekoppeltem Viskosimeter erhaltenen MePwerte hinsichtlich der axialen DispersionDie Korrektur der an Polystyrenproben mittels eines Gelpermeationschromatographen mit on-line-Viskosimeter erhaltenen Analysenergebnisse wurde unter Verwendung eines Verfahrens dnrchgefiihrt, das in einer vorausgegangenen hrbeit beschrieben wurde. Die von den beiden Detektoren gelieferten Werte werden getrennt korrigiert, anstelle einer Korrektur der Molekulmassen unter Verwendung der Angaben beider Detektoren. Damit besteht keine Notwendigkeit, Systeme von linearen Gleichungen zu losen, man kann vielmehr jede Methode anwenden, die zur Korrektur der Konzentrationskurve geeignet ist. I n dieser Arbeit ist es die Methode von PIERCE und ARMONAS. Ilonpasica na ocesoe paccesnue aannbtx zem-nponuiEamzyeiL xpowamozpaguu c nocJceaymqe6 sucicoauwempueiLn o n p a m a H a oceBoe paccemme p e 3 y n b~a r o~ ~H~J I H~O B 0 6 p a s s o~ nonucTupona H a xpoMaTorpa4e AJIH r e a m p o m -KaIOKI(er0 aHaJIH3a (mx) C nPHC0eAHHeHHHM BHCKOBHMeTPOM 6una CAeJIaHa C HCnOJIb30BaHHeM MeTOAa, ripen-JIOXeHHOrO B IIpe,QIIIeCTBylO~e8 pa6ore. nOIIpaBKy H e BHOCHJIM B 3HaqeHHR MOJIeKyJIfIpHbIX MaCC, BbI'IHCJIeHHbIe H a OCHOBaHHH AaHHbIX o6onx AeTeKTOpOB annapaTypbI, K a K 3 T 0 0 6~9~0 AeJIaeTCH, a B AaHHbIe KaH(AOr0 H3 ABYX AeTeKTOpOB annapaTypbI B OTAeJIbHOCTH. B TaHOM CJIysae HeT HeO6XOAHMOCTH nOJIb30BaTbCH MeTOAaMH, OCHOBaHHbIMH H a PeIIIeHHH CHCTeM JIHHefiHbIX ypaBHeHH8 H K a K IIpaBHJIO He AaIoKI(HMH XOPOIIIHX pe3yJIbTaTOB, HO M O X H O IICIIOJIb-30BaTb ~110608 MeTOR, IIepBOHaqaJIbHO pa3pa60TaHHbIfi TOJIbKO AJIR BHeCeHHFi IIOnpaBKE B 3anECb KOHqeHTpaqEE, B HameM cnyqaa MeTOA nBPCA H APMOHACA. h k -lhk D k -l -D k ' h k + l = h k -D& 58 66 W 82 90 sif 98 V Pig. 2. Comparison between the calibration dependence (straight line) of the column the log d? vs y dependence determined from the data of the concentration and viscometric detectors of the GPC apparatus corrected for axial dispersion NETOPIL~K and SCHULZ: Correction for axial dispersion of data obtained by gel permeation chromatography 1220 1180 665 190 Acta Polymerica 35 (1984) Nr. 2 143 M , = 1.35 x 10' (LS) M , = 1.19 x l o 6 (LS) M , = 8.78 : 105 (LS) M , = 8.31 x 104 (0s) Table 1. Unfractionated samples. M , was calculated using K =0.039, a = 0.58 1 2 3 4 132 130 93 45 Table 2. Results of GPC ana...

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Method for correcting molecular weight from gel‐permeation chromatography. I. Skewing and concentration corrections

Cited by 11 publications

References 10 publications

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Correction for axial dispersion of data obtained by gel permeation chromatography with an on‐line viscometer

Correction for axial dispersion in gel permeation chromatography with a detector of molecular masses

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