Method for contamination of filtering facepiece respirators by deposition of MS2 viral aerosols

Abstract

2016-12-24T18:13:07

Collection and measurement of aerosols of viable influenza virus in liquid media in an Andersen cascade impactor

“…An added complication is that viruses are often damaged/inactivated during collection using air samplers, due to the stresses and forces acting upon them. 24,[28][29][30][31] To be able to evaluate and compare different methods for detecting viable viral aerosols, our first goal was to develop a system that could safely, efficiently, and consistently generate aerosolized influenza virus. The viral aerosol-generation system was coupled with a bioaerosol sampler for characterizing airborne particles associated with viable influenza viruses.…”

Airborne Virus Survivability During Long-Term Sampling Using a Non-Viable Andersen Cascade Impactor in an Environmental Chamber

“…The plots show that MS2 generally had higher relative recovery and survival at lower RH or AH. In previous studies, MS2 aerosol generally had the highest survival at <30% RH, the lowest survival near 60% RH, and medium survival at >60% RH (Dubovi and Akers 1970;Trouwborst and de Jong 1973;Woo et al 2010). In Appert et al (2012), MS2 was found to have the highest relative recovery at 50% compared with results at 15% and 85% using the same suspension as used in current study.…”

“…MS2 is a small (25 to 30 nm), non-enveloped, single-stranded RNA (ssRNA) coliphage. It has been frequently tested as a general surrogate for animal and human viruses due to its innocuousness, high stock titer, and rapid analysis (Woo et al 2010;Appert et al 2012). The high stock titer of MS2 compared to AIV and HAdV-1 used in this study gives MS2 an advantage for better virus detection and lower measurement uncertainty.…”

Evaluation of the Performance of Iodine-Treated Biocidal Filters Under the Influence of Environmental Parameters

“…Mucin-viscous glycoproteins comprising approximately 75% carbohydrate and 25% amino acids linked via glycosidic bonds between Nacetylgalactosamine and serine or threonine residues-readily forms a gel in water. Details of AS composition were reported by Woo et al (2010).…”