An eco-friendly liquid chromatography method was developed for determining imatinib mesylate in
pharmaceuticals. During pre-development studies, Using a greener mobile phase and a combination of
qualitative and semi-quantitative green metrics techniques, the environmental sustainability of the
procedure was maintained. Methanol:water (pH maintained at 3.5 using orthophosphoric acid) at 75:25%,
v/v flowing at 1 mL/min was the final mobile phase used on a C-18 column. The diode array detector
detected imatinib at 262 nm. Method specificity, accuracy, precision, selectivity and system suitability
were tested over a linear concentration range of 5-120 μg/mL of imatinib. The limit of detection and
limit of quantitation values were 1.5 and 5.0 μg/mL, respectively. In addition, the factorial design
showed the procedure to be reliable with minimum testing. Finally, the method recovered the maximum
(> 99%) of the analyte from the dosage form. Overall, the present method is suitable for routine
applications in quality control of dosage forms of imatinib.