Method development for accurate determination of eight polycyclic aromatic hydrocarbons in extruded high-impact polystyrene

Baek, Song-Yee; Lim, Dong Kyu; Han, Jeesoo; Lee, Sun Young; Kim, Byung-Joo

doi:10.1016/j.chemosphere.2021.129909

Cited by 5 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The following supporting information can be downloaded at https: //www.mdpi.com/article/10.3390/toxics12020161/s1, Figure S1: The cell viability of KRISS CRM 109-02-004 and NIST SRM 1648a; Figure S2: Cellular ROS were induced by NIST SRM in BEAS-2B cells; Figure S3: The mRNA expression level of Keap1; Table S1: Certified values of KRISS CRM 109-02-004, urban particulate matter; Table S2: Approximate contents of elements in KRISS CRM 109-02-004, urban particulate matter; Table S3: The primer sequence of genes used in RT-qPCR. References [125][126][127][128] Funding: This research was supported by the "Establishment of measurement standards for Chemistry and Radiation" and "Establishing national metrology infrastructure for reliable measurement of particulate matter", grant number KRISS-2024-GP2024-0006 and GP2024-0009, respectively, funded by the Korea Research Institute of Standards and Science.…”

Section: Supplementary Materialsmentioning

confidence: 99%

“…The following supporting information can be downloaded at , Figure S1: The cell viability of KRISS CRM 109-02-004 and NIST SRM 1648a; Figure S2: Cellular ROS were induced by NIST SRM in BEAS-2B cells; Figure S3: The mRNA expression level of Keap1; Table S1: Certified values of KRISS CRM 109-02-004, urban particulate matter; Table S2: Approximate contents of elements in KRISS CRM 109-02-004, urban particulate matter; Table S3: The primer sequence of genes used in RT-qPCR. References [ 125 , 126 , 127 , 128 ] are cited in the Supplementary Materials.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells

Ahn,

Yim,

Yoo

2024

Toxics

View full text Add to dashboard Cite

Numerous toxicological studies have highlighted the association between urban particulate matter (PM) and increased respiratory infections and lung diseases. The adverse impact on the lungs is directly linked to the complex composition of particulate matter, initiating reactive oxygen species’ (ROS’) production and consequent lipid peroxidation. Excessive ROS, particularly within mitochondria, can destroy subcellular organelles through various pathways. In this study, we confirmed the induction of ferroptosis, an iron-dependent cell death, upon exposure to an urban PM using RT-qPCR and signaling pathway analysis. We used KRISS CRM 109-02-004, the certified reference material for the analysis of particulate matter, produced by the Korea Research Institute of Standards and Science (KRISS). To validate that ferroptosis causes lung endothelial toxicity, we assessed intracellular mitochondrial potential, ROS overproduction, lipid peroxidation, and specific ferroptosis biomarkers. Following exposure to the urban PM, a significant increase in ROS generation and a decrease in mitochondrial potential were observed. Furthermore, it induced hallmarks of ferroptosis, including the accumulation of lipid peroxidation, the loss of antioxidant defenses, and cellular iron accumulation. In addition, the occurrence of oxidative stress as a key feature of ferroptosis was confirmed by increased expression levels of specific oxidative stress markers such as NQO1, CYP1B1, FTH1, SOD2, and NRF. Finally, a significant increase in key ferroptosis markers was observed, including xCT/SLC7A11, NQO1, TRIM16, HMOX-1, FTL, FTH1, CYP1B1, CHAC1, and GPX4. This provides evidence that elevated ROS levels induce oxidative stress, which ultimately triggers ferroptosis. In conclusion, our results show that the urban PM, KRISS CRM, induces cellular and mitochondrial ROS production, leading to oxidative stress and subsequent ferroptosis. These results suggest that it may induce ferroptosis through ROS generation and may offer potential strategies for the treatment of lung diseases.

show abstract

Section: Supplementary Materialsmentioning

confidence: 99%

mentioning

confidence: 99%

Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells

Ahn,

Yim,

Yoo

2024

Toxics

View full text Add to dashboard Cite

show abstract

“…Moreover, it has the characteristics of structure–activity prediction, specific recognition and wide applicability. Therefore, it is widely used in the fields of miniaturization sample preparation and separation, 8 detection of organic pollutants, 9 microbial analysis, 10 clinical diagnosis 11 and others. However, MIPs prepared by traditional methods have some limitations, such as low efficiency of the sample pretreatment, few effective imprinting cavities and low molecular weight of the template.…”

Section: Introductionmentioning

confidence: 99%

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

Wang

Yang

Pang

et al. 2022

Analyst

View full text Add to dashboard Cite

show abstract

Gas Chromatography in Analysis of Polymers and Rubbers

Hakkarainen

Karlsson

2021

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

This article gives an overview of gas chromatography (GC) and gas chromatography–mass spectrometry (GC‐MS) of low molar mass compounds present in or migrating from polymers and rubbers. GC is a powerful tool for the analysis of volatile and semi‐volatile organic compounds. The capability of GC to separate and quantify small amounts of analytes makes it highly applicable for the analysis of low molar mass compounds in polymers, such as residual monomers, initiators, catalysts, additives, and degradation products. Correctly chosen and performed sample preparation is a key step before GC‐MS analysis and crucial for obtaining correct results. This can be especially challenging when analyzing low molar mass compounds in solid matrices or in complex aqueous solutions. Some commonly applied extraction techniques are shortly presented including extraction techniques that can be utilized for extraction of low molar mass compounds from solid and aqueous matrices as well as air. Last but not least, examples of extraction and analysis of low molar mass compounds present in, formed in, or migrating from different polymeric materials during processing, service‐life, or reprocessing (recycling) are given with special focus on packaging and medical applications as well as degradable polymers.

show abstract

Method development for accurate determination of eight polycyclic aromatic hydrocarbons in extruded high-impact polystyrene

Cited by 5 publications

References 16 publications

Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells

Particulate Matter Induces Oxidative Stress and Ferroptosis in Human Lung Epithelial Cells

Application progress of magnetic molecularly imprinted polymers chemical sensors in the detection of biomarkers

Gas Chromatography in Analysis of Polymers and Rubbers

Contact Info

Product

Resources

About