Method Development and Validation of Ephedrine and Pseudoephedrine in Horse Urine by Liquid Chromatography-Tandem Mass Spectrometry

Maurya, Ashok Kumar; Sahu, Puran Lal; Jethi, Sakshi; Gosain, Himank; Sahu, Kapendra

doi:10.5530/ijper.54.3s.167

Cited by 2 publications

(1 citation statement)

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“…However, these methods have some limitations, given the complicated sample processing procedure and low sensitivity. In addition, previous studies have described liquid chromatography with tandem mass spectrometry detection (LC-MS/MS), which was reportedly simpler and more sensitive for analyzing six ephedra alkaloids in dietary supplements and biological fluids [16], as well as ephedrine and pseudoephedrine in rat plasma and horse urine [17][18][19]. LC-MS/MS was employed for determining nine herbal phenalkylamines in human plasma, including ephedrine and pseudoephedrine [20].…”

Section: Introductionmentioning

confidence: 99%

A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma

et al. 2021

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A sensitive and reproducible liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and fully validated for the simultaneous determination of ephedrine and pseudoephedrine in human plasma after oral administration of the herbal prescription Ojeok-san (OJS); 2-phenylethylamine was used as the internal standard (IS). Both compounds presented a linear calibration curve (r2 ≥ 0.99) over a concentration range of 0.2–50 ng/mL. The developed method was fully validated in terms of selectivity, lower limit of quantitation, precision, accuracy, recovery, matrix effect, and stability, according to the regulatory guidelines from the U.S. Food and Drug Administration and the Korea Ministry of Food and Drug Safety. This validated method was successfully applied for the pharmacokinetic assessment of ephedrine and pseudoephedrine in 20 healthy Korean volunteers administered OJS.

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