Method development and validation for simulataneous estimation of albendazole and praziquantel in bulk and in a synthetic mixture

Abstract

A simple, rapid, sensitive reversed-phase high-performance liquid chromatography method was developed and validated for simultaneous measurement of albendazole and praziquantel with an internal standard, simvastatin, at single wavelength of 225 nm. Chromatographic separation was performed on an Enable C 18 column (250 mm × 4.6 mm, 5 m: Spinco Biotech Pvt Ltd) and a mobile phase consisting of acetonitrile:water (60:40, v/v) with 10% orthophosphoric acid to adjust the pH to 3.2, at a flow rate of 1.0 ml/min. The calibration curve was linear (r 2 ≥ 0.999) over the concentration range 0.05-8.0 g/ml. The concentrations of simvastatin was 1.0 g/ml. The limit of quantification was 0.05 g/ml for both albendazole and praziquantel. No interference was found by the excipients in the synthetic mixture. The proposed methods were validated as per International Conference on Harmonisation guidelines for linearity, accuracy, precision and robustness for estimation of albendazole and praziquantel in bulk and in a synthetic mixture, and the results were found to be satisfactory.

Bioavailability of Albendazole and its Metabolities in Plasma of Pangasianodon hypophthalmus with High Performance Liquid Chromatography

“…In this study calibration graphs containing in the range of 0.05 to 10 ugml-1 of ABZ, ABZSO and ABZSO2 with calibration curves were linear (r2 ≥ 0.990) which is similarly reported by (Shah et al, 2014;Shaikh et al, 2003). A 20 mg single dosage was given through oral intubation, intramuscular and intraperitoneal, the drug was found in oral dose only.…”

Method development and validation for dieckol in the standardization of phlorotannin preparations

“…Linearity is the ability to elicit results through the analysis of diverse concentrations within a given range (Shabir, 2003;Shah et al, 2014). A linear regression analysis (n = 3) was measured over seven different concentrations (12.5-400 μg/mL), and its regression equations are given in Table 2.…”

Degradation Kinetics, <i>In Vitro</i> Dissolution Studies, and Quantification of Praziquantel, Anchored in Emission Intensity by Spectrofluorimetry

“…1,2 An extended literature survey revealed that few analytical methods [using high-performance liquid chromatography (HPLC) and HPLC-mass spectrometry] have been reported for estimation of praziquantel. [3][4][5][6][7] To date, one spectrofluorimetric method has been reported for praziquantel quantification in blood plasma and urine. However, the method was complicated because of drug chemical derivatization with dansyl chloride, which involved various solvents with many extraction procedures.…”