Method Development and Validation for Simultaneous Estimation of Cefpodoxime Proxetil and Dicloxacillin Sodium by Hydrotropy Using UV Spectroscopy

Abstract

The present work describes the development of accurate, precise and high economic value for the simultaneous estimation of Cefpodoxime proxetil and Dicloxacillin sodium by hydrotropy method using UV spectroscopy was developed and validated. The method was performed on Lab c, model UV-3000 spectrophotometer with UV win software. 1M urea was used as a solvent for analysis. Detection was carried out at 230nm for Cefpodoxime proxetil and 210 nm for Dicloxacillin sodium. Linearity was observed at concentration range 2-20 µg/ml for Cefpodoxime proxetil and 5-30 µg/ml for Dicloxacillin sodium. Correlation coefficient for Cefpodoxime proxetil and Dicloxacillin sodium was found to be 0.993 and 0.9998 respectively. The method can be successfully applicable to routine analysis.