Method and Electrochemical Biosensor for Detection of Copper in Wine

Abstract

The developed biosensor is based on the electrochemical signal of the complex formed between the ligand used (glycine) and copper(II) ions. The developed electrochemical method is quick, inexpensive, easy to apply, and has shown good selectivity for copper ions. In this research, a linear response for copper ions was obtained in the concentration range of 0.2 mg to 1 mg/L, which largely covers the area of copper ion concentrations in wine. The achieved sensitivity was 11.05E-05 and the limit of detection (LOD) was 0.041 ppm. The biosensor with screen printed electrodes has been successfully used to detect copper ions in wine. A comparative study between the developed portable biosensor and ICP-MS analysis results of the same samples showed similar results.

An overview of Structured Biosensors for Metal Ions Determination

“…The association of this strategy with electrochemical transducers significantly lowered the detection limits, especially when stripping voltammetry was employed for the determination of elements [17,46,47]. The coordination of metal ions with proteins [74], aminoacids [17,[75][76][77][78][79], synthetized peptides [70], hormones (melatonine [80] and oxytocin [7]), DNA strands [17,46,[81][82][83], and a neurotransmitter (neurokinin B [38]) were exploited to achieve detection limits as low as 0.80 ag L −1 [81]. The selected articles regarding this mechanism are displayed in Table 1.…”